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pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

Plasmid

#55218

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

Plasmid

#192184

Purpose

Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

Plasmid

#192183

Purpose

Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

Plasmid

#192185

Purpose

Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

Plasmid

#55219

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6

Expression

Insect

Mutation

Promoter

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6 and MBP, Asn10

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pEPIP_Peptide_Cloning_Vector

Plasmid

#172110

Purpose

Lentiviral plasmid for protein / peptide overexpression

Depositor

Prashant Mali

Article

Ford et al Cell Syst. 2021 Jul 21;12(7):716-732.e7.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ef1-alpha

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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XClone_Cas9_KRAB_T2A_BFP

Plasmid

#141208

Purpose

Tunable and temporal expression control of dCas9

Depositor

Rory Johnson

Insert

dCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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psglessClonePX

Plasmid

#87193

Purpose

used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pPEPZ-sgRNAclone

Plasmid

#141090

Purpose

This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

Depositor

Jan-Willem Veening

Article

Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

Insert

mCherry

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

p3

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone76)

Plasmid

#188579

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Use

Tags

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Use

Tags

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDL52 (clone45)

Plasmid

#188578

Purpose

High-yield production of coenzyme F420 in E. coli

Depositor

Gerald Lackner

Article

Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

Inserts

ribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE

Use

Tags

Expression

Bacterial

Mutation

Codon optimization, synthetic ribosome binding si…

Promoter

T7 variants

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Tags

Expression

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Promoter

Available since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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pUC57-BBclone1

Plasmid

#137069

Purpose

E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0405 (BB_0405 Borrelia burgdorferi B31)
BB0406
P66
BBA57
HtrA
RevA (revA Borrelia burgdorferi B31)
BB0238

Use

Tags

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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