Addgene

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AAA

Plasmid

#78487

Purpose

2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail

Depositor

Allan Jacobson

Article

Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d

Insert

CAN1-LUC

Use

Tags

Expression

Bacterial

Mutation

CAN1 contains no stop codon

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only
pLNHA-C1-HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres_AC

Plasmid

#148442

Purpose

Mammalian Expression of HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1-FNKRNFKFEtoAAAAAAAAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

Plasmid

#148404

Purpose

Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-YYMYFWFtoAAAAAAA_AH

Plasmid

#148941

Purpose

Insect Expression of DmGIGYF1-YYMFWFtoAAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-YYMFWFtoAAAAAAA (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-QHTKRKKtoAAAAAAA_AC

Plasmid

#148396

Purpose

Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTKRKKtoAAAAAAA (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC

Plasmid

#148386

Purpose

Insect Expression of DmMe31B-QHTRKKtoAAAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTRKKtoAAAAAA (me31B Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres_AC

Plasmid

#148390

Purpose

Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres (me31B Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC

Plasmid

#148452

Purpose

Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRRKKtoAAAAAAA-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

Plasmid

#148910

Purpose

Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-PPFPPFtoAAAAAA_AH

Plasmid

#148908

Purpose

Insect Expression of DmHPat-PPFPPFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmHPat-PPFPPFtoAAAAAA (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

three silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

Plasmid

#169126

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Use

Tags

Expression

Mammalian

Mutation

NAWLVH106-AAAAAA

Promoter

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-YMCLLtoAAAAA_Z

Plasmid

#148194

Purpose

Bacterial Expression of DmThor-YMCLLtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-YMCLLtoAAAAA (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-YMCLLtoAAAAA_Z

Plasmid

#148153

Purpose

Insect Expression of DmThor-YMCLLtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-YMCLLtoAAAAA (Thor Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-LLFWKtoAAAAA_Z

Plasmid

#148156

Purpose

Insect Expression of Dm4E-T-LLFWKtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T-LLFWKtoAAAAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

one non silent mutation L127S, annotated as EST, …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMextli-YLLILtoAAAAA_AB

Plasmid

#148348

Purpose

Insect Expression of DmMextli-YLLILtoAAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli-YLLILtoAAAAA (mxt Fly)

Use

Tags

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-AAAAA-nCas9(D10A)-UGI

Plasmid

#187409

Purpose

DddA AAAAA variant

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA AAAAA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmMextli_577-640-YLLILtoAAAAA-GB1_AB

Plasmid

#148366

Purpose

Bacterial Expression of DmMextli_577-640-YLLILtoAAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli_577-640-YLLILtoAAAAA (mxt Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-LLFWKtoAAAAA-V5His6_Z

Plasmid

#148160

Purpose

Insect Expression of Dm4E-T_1-511-LLFWKtoAAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-LLFWKtoAAAAA (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

alias: 4E-T; one non silent mutation L127S, annot…

Promoter

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFUSB4_AAAA

Plasmid

#48368

Depositor

Stephen Ekker

Article

Ma et al PLoS One. 2013 May 29;8(5):e65259. doi:

Insert

RVD sequence: NI NI NI NI

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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