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Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
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LentiSAMPHv2
Plasmid#167934PurposeAll-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorInsertTmpo (Tmpo Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSPORT-RREB1
Plasmid#41145DepositorInsertRREB-1 (RREB1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEL685
Plasmid#205592PurposeNEPE for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL682
Plasmid#205591PurposePE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL683
Plasmid#205593PurposeCas9-PE2 for prime editing in riceDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL686
Plasmid#207510PurposeePPE for prime editing in riceDepositorInsertzCas9 (H840A)-NC-M-MLV RT-deltaRH
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterZmUbiAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M4
Plasmid#203320PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hMuc18 fused to Ig like C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M2
Plasmid#203318PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hMuc18 fused to Ig like C1-C3 of hB…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M1
Plasmid#203317PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hMuc18 fused to Ig like V2-C3 of hBCAMPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B4
Plasmid#203316PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C2 of hBCAM fused to Ig like C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B3
Plasmid#203315PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hBCAM fused to Ig like C2-C3 of hMUC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B1
Plasmid#203313PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1 of hBCAM fused to Ig like V2-C3 of hMUC18PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-M3
Plasmid#203319PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthBCAM/hMUC18
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBC…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
hBCAM-B2
Plasmid#203314PurposeOverexpression of BCAM-MCAM chimerasDepositorInserthMUC18/hBCAM
UseTagsFlag (DYKDDDK)ExpressionMammalianMutationIgG V1-V2 of hBCAM fused to Ig like C1-C3 of hMU…PromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pM-CBE
Plasmid#196293Purpose35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GPDepositorInsertFull length TSWV M antigenome encoding CBE in place of viral GP
UseCRISPRTags3×flagExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-cj-E sgRNA
Plasmid#169915PurposeExpression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only