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We narrowed to 33 results for: 34879

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pCAT202

Plasmid

#134879

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

deletion of Y208

Promoter

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTV-Gal4DBD2xHighMag1

Plasmid

#182023

Purpose

Donor plasmid for conversion of Gal4 line into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1

Use

Donor plasmid

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRIV-Gal4DBD:2xnMagHigh1

Plasmid

#182031

Purpose

Donor plasmid for conversion of KO attP Drosophila lines into ShineGal4 drivers

Depositor

Jean-Paul Vincent

Article

di Pietro et al Dev Cell. 2021 Dec 20;56(24):3393-3404.e

Insert

Gal4DBD2xHighMag1-p103'UTR

Use

Donor plasmid

Tags

Expression

Insect

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-Cys-HA-Ubiquitin

Plasmid

#185356

Purpose

In vitro ubiquitination assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ubiquitin

Use

Tags

GST, HA(x2)

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRSCR

Plasmid

#185345

Purpose

Expression of soluble G2BR with scrambled sequence

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

scrambled gp78 G2BR sequence

Use

Tags

EGFP

Expression

Mammalian

Mutation

scrambled sequence

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Use

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4-RI1-332-MYC-His6

Plasmid

#185351

Purpose

Mammalian expression of Ribophorin I

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Ribophorin I (RPN1 Human)

Use

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

aa 1-332 only

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG

Plasmid

#185336

Purpose

Mammalian expression of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-TRC8 513-664

Plasmid

#185352

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

TRC8 (RNF139 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 513-664 only

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-UBE2J1 1-283

Plasmid

#185348

Purpose

In vitro transcription and translation in bacterial system

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

UBE2J1 (UBE2J1 Human)

Use

Tags

Expression

Bacterial

Mutation

Cytoplasmic domain only, aa 1-283

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

Plasmid

#185346

Purpose

Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

Plasmid

#185347

Purpose

Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

Cue1p/Ube2G2 fusion (internal MYC tag)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG 5E

Plasmid

#185341

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

R382E/Q383E/K390E/R398E/R400E

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 292-410

Plasmid

#185331

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 292-410 only

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCINeo-gp78 Mutant G2BR

Plasmid

#185355

Purpose

Assessing cellular requirement for G2BRgp78

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

gp78 (AMFR Human)

Use

Tags

GST

Expression

Mammalian

Mutation

aa 595-600 KRFLNK to AGAAGG

Promoter

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 AUP1 61-410

Plasmid

#185334

Purpose

Bacterial expression of AUP1 with GST fusion protein for binding assays

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 61-610 only

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1 A397D

Plasmid

#185342

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

A397D

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRAUP1

Plasmid

#185343

Purpose

Expression of soluble G2BR, amino acids 378-404 of human AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 378-404 (G2BR) (AUP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 378-404 only

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG DTM

Plasmid

#185337

Purpose

Assessing domain requirements of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 24-45, corresponding to the hairpi…

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG-DATDCUE

Plasmid

#185340

Purpose

Assessing domain requirement sof AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

deletion of aa 90-337 corresponding to the acyl t…

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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