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Showing: 1 - 40 of 76 results

  1. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  10. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  11. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

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  20. pJK635

    Plasmid
    #72473
    Purpose
    pET28a modified to contain TEV protease fusion tag instead of T7 tag
    Depositor
    T. J. Kappock
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72473-plasmid-map-sequence-id-132656

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  21. pOPINB

    Plasmid
    #41142
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41142-plasmid-map-sequence-id-54742

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  22. pOPINA

    Plasmid
    #41141
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41141-plasmid-map-sequence-id-54739

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  23. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208053-plasmid-map-sequence-id-415923

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  24. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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  25. pOPINK

    Plasmid
    #41143
    Depositor
    Ray Owens
    Insert
    GST
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41143-plasmid-map-sequence-id-54743

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  26. p28BIOHTEV-LIC

    Plasmid
    #167852
    Purpose
    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167852-plasmid-map-sequence-id-331065

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  27. pET28GSTlinker-LIC

    Plasmid
    #119970
    Purpose
    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119970-plasmid-map-sequence-id-232695

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  28. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  29. TBXTA-c005

    Plasmid
    #139754
    Purpose
    For protein expression/crystallography (PDB:6F58)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139754-plasmid-map-sequence-id-296505

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  30. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  31. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Melissa Jurica, Tara Davis
    Insert
    ppie (PPIE Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  32. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

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  34. pET28-GG-PUF

    Plasmid
    #52137
    Purpose
    Receiving vector for PUM-HD assembly and expression in E.coli
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Type
    Empty backbone
    Use
    Tags
    PUM-HD flanking region 1' and PUM-HD flankin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    52137-plasmid-map-sequence-id-80978

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  35. G392A FL USP7

    Plasmid
    #227217
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392A
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227217-plasmid-map-sequence-id-452329

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  36. L469R USP7 catalytic

    Plasmid
    #227212
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L469R; aa 207-560
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227212-plasmid-map-sequence-id-452328

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  37. N418S FL USP7

    Plasmid
    #227215
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    N418S
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227215-plasmid-map-sequence-id-449605

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  38. G392D FL USP7

    Plasmid
    #227216
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    G392D
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227216-plasmid-map-sequence-id-449551

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  39. K420E FL USP7

    Plasmid
    #227223
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    K420E
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227223-plasmid-map-sequence-id-449550

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  40. L299A USP7 catalytic

    Plasmid
    #227213
    Purpose
    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    L299A; aa 207-560
    Promoter
    T7-promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    227213-plasmid-map-sequence-id-449549

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Showing: 1 - 40 of 76 results