-
Plasmid#217341PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertsUseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only
-
gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217342PurposecrRNA array targeting CD81, B2M, KIT, CD55DepositorInsertscrCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)
Plasmid#217345PurposecrRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81DepositorInsertscrCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-WT-V5
Plasmid#196018PurposeExpresses CRKL WT, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pHH0103_CRKL-2/2
Plasmid#91364PurposeProtein expression and purification of human SH3 domain construct CRKL-2/2DepositorInsertCRKL-2/2 (CRKL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCRK_SH3 (CRK Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28P_dCrk
Plasmid#131145PurposeExpresses 6XHis::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pLenti6.3_CRKL-Y207F-V5
Plasmid#196016PurposeExpresses CRKL Y207F, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl
Plasmid#36400DepositorInsertCrkl (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CRKL
Plasmid#23354DepositorInsertCRKL (CRKL Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGFP CrkII
Plasmid#50730Purposemammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertCrkII (CRK Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CCRK
Plasmid#23717DepositorInsertCCRK (CDK20 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-2_dCrk
Plasmid#131146PurposeExpresses GST::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CrkII
Plasmid#50731Purposemammalian expression of CrkIIDepositorInsertCrkII (CRK Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-2/2-SV1/2
Plasmid#91319PurposeProtein expression and purification of human SH3 domain construct CRK-2/2-SV1/2DepositorInsertCRK-2/2-SV1/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-2/2-SV2/2
Plasmid#91343PurposeProtein expression and purification of human SH3 domain construct CRK-2/2-SV2/2DepositorInsertCRK-2/2-SV2/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBB c-Crk II
Plasmid#15000DepositorInsertCrkII (CRK Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR-CrkII
Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b_CrK_amilCP
Plasmid#204067PurposeType TU2-b backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A_CrK_amilCP
Plasmid#204046PurposeType TU1-A backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzl_CRKL-W275L
Plasmid#196019PurposeExpresses CRKL W275LDepositorInsertCRKL
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-silent-V5
Plasmid#196015PurposeExpresses CRKL-silent, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-W160L-V5
Plasmid#196017PurposeExpresses CRKL W160L, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
plx304_CRKL-open-V5
Plasmid#196023PurposeExpresses CRKL open, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA2
Plasmid#131140PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRK7A-c021
Plasmid#53643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCRK7A
UseBaculovirus expressionTagsHis6-TEVExpressionMutationPromoterpolyhedrinAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSG5-CRKL-Y198FY207F
Plasmid#32506DepositorInsertCRKL (CRKL Human)
UseTagsExpressionMammalianMutationY207F and Y198FPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and tdTomatoExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Crk-SH2
Plasmid#46418DepositorInsertCrk (CRK Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 1-124PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH3SH3
Plasmid#36403DepositorInsertCrkl tandem SH3 domains (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRKL gRNA (BRDN0001145388)
Plasmid#77508Purpose3rd generation lentiviral gRNA plasmid targeting human CRKLDepositorInsertCRKL (CRKL Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRKL gRNA (BRDN0001148341)
Plasmid#77509Purpose3rd generation lentiviral gRNA plasmid targeting human CRKLDepositorInsertCRKL (CRKL Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH2
Plasmid#36402DepositorInsertCrkl SH2 domain (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only