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Plasmid#160384PurposeFor CRISPR-mediated deletin og MAK10 in yeastDepositorInsertMAK10
UseTagsExpressionYeastMutationPromoterPGK1Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MAK
Plasmid#23624DepositorInsertMAK (MAK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK461
Plasmid#140699PurposeNBαRb-IgG-FcDepositorInsertNBαRb-IgG-Fc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK462
Plasmid#140700PurposeNBαMs/Rt-IgG1-FcDepositorInsertNBαMs/Rt-IgG1-Fc
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
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786 pCSmAKT2Acherry
Plasmid#31167DepositorInsertAkt
UseExpression vectorTagsMyr and mCherryExpressionMutationmembrane tethered AktPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mAkt-ER
Plasmid#39530DepositorInsertAkt (Akt1 Mouse)
UseTagsERExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Mak11
Plasmid#63761Purposeyeast ORF Mak11 in the Gateway entry vector pDONR221DepositorInsertMak11
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Mak16
Plasmid#63762Purposeyeast ORF Mak16 in the Gateway entry vector pDONR221DepositorInsertMak16
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUG-amdSYM-mak10TR
Plasmid#75433PurposeGenerates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiaeDepositorInsertsMAK10TR
ApR
amdSYM
UseTagsExpressionYeastMutationN-terminal 740 bp of MAK10 ORFPromoterNative promoter and PTEFAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMaKIIalpha-mKO2
Plasmid#86156PurposeLentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.DepositorInsertmKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA
Plasmid#160385PurposeExpresses guide RNA for targeting MAK10 in yeastDepositorInsertmak10 gRNA
UseTagsExpressionYeastMutationPromoterRNA pol III promoter (tRNA-Tyr)Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAK gRNA (BRDN0001148291)
Plasmid#76709Purpose3rd generation lentiviral gRNA plasmid targeting human MAKDepositorInsertMAK (MAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAK gRNA (BRDN0001146769)
Plasmid#76710Purpose3rd generation lentiviral gRNA plasmid targeting human MAKDepositorInsertMAK (MAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAK gRNA (BRDN0001145227)
Plasmid#76711Purpose3rd generation lentiviral gRNA plasmid targeting human MAKDepositorInsertMAK (MAK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-GST-Presc-mAkt1 (wt)
Plasmid#48805Purposeexpresses wt Akt1DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA-GST-PrescExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol1-uas-mAKT-2a-nlstdTomato
Plasmid#67706PurposeTol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomatoDepositorInsertmAKT-2A-nlstdTomato
UseTagsExpressionMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-mAK1-shRNA-1
Plasmid#185364PurposeFor mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)DepositorInsertAK1 (Ak1 Mouse)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only