Addgene

Showing: 141 - 160 of 7821 results

Results per page:

Sort results by:

LOV-Turbo-V5_pRS415

Plasmid

#199667

Purpose

expresses LOV-Turbo in the yeast cytosol

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Tags

V5

Expression

Yeast

Mutation

Promoter

Gal1,10

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB418

Plasmid

#185088

Purpose

Swi6L-GFP with nuclear export signal mutated to alanines

Depositor

Ken Belanger

Article

Belanger et al BMC Mol Cell Biol. 2022 Feb 21;23(1):10.

Insert

SWI6

Use

Tags

GFP

Expression

Yeast

Mutation

Swi6 nuclear export signal L to A mutations

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KBB460

Plasmid

#185094

Purpose

NMA111-GFP wild type under endogenous promoter

Depositor

Ken Belanger

Article

Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

Insert

NMA111

Use

Tags

GFP

Expression

Yeast

Mutation

NMA111-GFP

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Use

Tags

NubI

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUM

Plasmid

#64176

Purpose

harbours marker genes URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pROS17

Plasmid

#107931

Purpose

TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYDS2.0_AT118-H

Plasmid

#222846

Purpose

Yeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-H

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p406TEF1

Plasmid

#15976

Depositor

Nicolas Buchler, Fred Cross

Insert

TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

TEF1 promoter is between SacI and XbaI restrictio…

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pE5n

Plasmid

#17459

Purpose

modified pENTR vector for N-terminal ECFP tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

ECFP

Expression

Bacterial, Insect, Mammalia…

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-GW

Plasmid

#61703

Purpose

Gateway compatible Y2H vector - BD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-T7-GFP

Plasmid

#33130

Depositor

Michael Rosbash

Article

Dower et al RNA. 2002 May;8(5):686-97.

Insert

GFP with TDH3 5' and 3' UTR (gfp )

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
PGAL

Plasmid

#60337

Purpose

Contains full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Inserts

promoter
yECitrine

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pPL5606_TEF1*-Cre_ADE2

Plasmid

#60932

Purpose

Constitutive expression vector for Cre-recombinase containing ADE2 selection marker.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Cre

Use

Tags

Driven from a mutant TEF1 promoter with 0.16x act…

Expression

Yeast

Mutation

Promoter

mutant TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-HDEL

Plasmid

#129098

Purpose

For labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDEL

Depositor

Wei-Lih Lee

Article

Omer et al Elife. 2018 Aug 7;7. pii: 36745. doi: 10

Insert

TEF1p-SS-3xGlyAla-GFP-HDEL

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT302

Plasmid

#41845

Depositor

Dane Wittrup

Article

Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

Insert

4-4-20

Use

Tags

HA and c-myc

Expression

Yeast

Mutation

Promoter

Available since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge