7,979 results

1. p413TEF-Sir2-H364Y

   Plasmid

   #51743

   Purpose

   overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   Sir2 (SIR2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H364Y

   Promoter

   TEF1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. p404-Grx5-GFP1-10

   Plasmid

   #91952

   Purpose

   Expresses GFP1-10 in the mitochondrial matrix

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   Grx5-GFP1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAP

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pI2-MTU-DO-G418

   Plasmid

   #160018

   Purpose

   Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-link-yomKeima-Kan

   Plasmid

   #44902

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomKeima
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Plasmid

   #58439

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2795

   Plasmid

   #63650

   Purpose

   pCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIPlac204TKC-E2-Crimson-HDEL

   Plasmid

   #38771

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Use

   Tags

   Linker peptide and yeast ER retention signal sequ…

   Expression

   Yeast

   Mutation

   Promoter

   TPI1

   Available since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pI6-MTU-DO-URA

   Plasmid

   #160030

   Purpose

   Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK3FS

    Plasmid

    #85776

    Purpose

    N-ICE plasmid pK3FS

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-KanMX-loxP-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG304GAL-ccdB

    Plasmid

    #14135

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. M4755 KanMX::LEU2 Disruptor Converter

    Plasmid

    #51685

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to LEU2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LEU2 (LEU2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEV-G2-Ph

    Plasmid

    #46816

    Purpose

    TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

    Depositor

    Lars Nielsen, Claudia Vickers

    Article

    Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

    Type

    Empty backbone

    Use

    Tags

    FLAG and c-myc

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 and PGK1

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYX122-mtGFP

    Plasmid

    #45048

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. M3925 trp1::KanMX3 Disruptor Converter

    Plasmid

    #51681

    Purpose

    Yeast marker swap plasmid for converting Trp1 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pGBW-m4046660

    Plasmid

    #149214

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGBW-m4046689

    Plasmid

    #149212

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pJJH1260

    Plasmid

    #36913

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Inserts

    ScURA3
    ScURA3
    

    Use

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIplac211-VPS8-HaloTagx2

    Plasmid

    #177113

    Purpose

    Expresses Vps8-HaloTagx2 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    VPS8 (VPS8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge