7,977 results

1. pBEVY-pT231 scFv-GFP

   Plasmid

   #218827

   Purpose

   Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

   Depositor

   Yongku Cho

   Article

   Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

   Insert

   pT231 tau scFv
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pESC/mCitrine

   Plasmid

   #117996

   Purpose

   Expression of mCitrine controlled by the Gal1 promoter

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   mCitrine
   

   Use

   Yeast/bacteria binary vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB364

   Plasmid

   #185083

   Purpose

   RFA1 fused to GFP under control of GAL1 promoter

   Depositor

   Ken Belanger

   Article

   Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

   Insert

   RFA1
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLH_Scr15

   Plasmid

   #100537

   Purpose

   CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

   Inserts

   Phytochrome B (PHYB Mustard Weed)
   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   CreC, CreN, and SV40 NLS

   Expression

   Yeast

   Mutation

   N-terminal version of PhyB (AA 1-621)/ Mutation o…

   Promoter

   FBA1 and TDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLexA::Ras

   Plasmid

   #11340

   Depositor

   Michael Wigler

   Article

   Chang et al Cell. 1994 Oct 7. 79(1):131-41.

   Insert

   Ras1 (ras1 Fission Yeast)
   

   Use

   Tags

   LexA DNA binding domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pCfB3045(gRNA XI-3)

   Plasmid

   #73287

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cip-pAct1-Mnase-SAT1

   Plasmid

   #162557

   Purpose

   For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

   Depositor

   Adnane Sellam

   Article

   Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

   Insert

   3FLAG-Mnase-SV40
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Actine

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIplac211-iGFP-TLG1

   Plasmid

   #105261

   Purpose

   Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Tlg1 (TLG1 Budding Yeast)
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-6xGLY-T7-hphMX4

   Plasmid

   #20761

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046708

    Plasmid

    #149173

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pNH605-CCAAT-YFP

    Plasmid

    #172132

    Purpose

    Fluorescent reporter for HAP complex activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xCCAAT-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of the H…

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG414GAL-ccdB

    Plasmid

    #14143

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Diethard Mattanovich, Brigitte Gasser, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. LPH2781 - Ub-K63R

    Plasmid

    #32160

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pAG303GPD-ccdB

    Plasmid

    #14134

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB4279

    Plasmid

    #98698

    Purpose

    A control plasmid containing the intact bsdMX marker

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG423GPD-EGFP-ccdB

    Plasmid

    #14318

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB2791

    Plasmid

    #63654

    Purpose

    pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDRF1 ymStayGold-T2A-mCherry

    Plasmid

    #219843

    Purpose

    equimolar expression of ymStayGold and mCherry in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymStayGold-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS415_pGal-nCas9(H840A)

    Plasmid

    #79616

    Purpose

    Expresses nCas9(H840A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H840A for nickase Cas9

    Promoter

    pGal1

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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