7,977 results

1. pHLUK

   Plasmid

   #64181

   Purpose

   harbours marker genes HIS3, LEU2, URA3 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-ymTurquoise2-CaURA3

   Plasmid

   #118458

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 426Gal-FUS

   Plasmid

   #29591

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pICXNHG3

   Plasmid

   #49021

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct::Raf

   Plasmid

   #11341

   Depositor

   Michael Wigler

   Article

   Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

   Insert

   byr-2 (byr2 Fission Yeast)
   

   Use

   Tags

   Gal4 activation domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

6. S3_001_026_MV2

   Plasmid

   #134422

   Purpose

   pYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZc-HsACTB*-thymosinB-8His

   Plasmid

   #111146

   Purpose

   Expresses human beta-actin fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   ACTB (ACTB Human)
   

   Use

   Pichia pastoris integration

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPS1622

   Plasmid

   #8854

   Depositor

   Pamela Silver

   Article

   Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

   Insert

   Sec63-sGFP (SEC63 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   sGFP=S65T V163A mutant of GFP

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-mRFP1-kanMX6

   Plasmid

   #133551

   Purpose

   Yeast fluorescent (RFP) vector with PTEF promoter.

   Depositor

   Won-Ki Huh, Erin O'Shea

   Article

   Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMM0638

    Plasmid

    #129005

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZJOM1

    Plasmid

    #133632

    Purpose

    Integration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-GFP-SED5 (SED5 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PhyB-mCherry-CAAX

    Plasmid

    #51567

    Purpose

    Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    CAAX and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWS176

    Plasmid

    #90963

    Purpose

    Cas9 gap repair vector - ZeoR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pDRF1 Cyterm-ytd8oxStayGold

    Plasmid

    #219853

    Purpose

    expression of Cyterm-ytd8oxStayGold in budding yeast

    Depositor

    Bas Teusink

    Insert

    Cyterm-ytd8oxStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pAG423GPD-ccdB-EGFP

    Plasmid

    #14198

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE3n

    Plasmid

    #17457

    Purpose

    modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV0133

    Plasmid

    #133467

    Purpose

    Integration into fission yeast genome at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEVY-4420-mCherry

    Plasmid

    #218828

    Purpose

    Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    4-4-20 scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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