7,977 results

1. pHCMM4

   Plasmid

   #170359

   Purpose

   Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter

   Depositor

   Marvin Wickens, Hugo Cesar Medina-Munoz

   Article

   Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   PCYC1_SEC61_mCherry_LEU2_TCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PCYC1

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIM573

   Plasmid

   #74637

   Purpose

   alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

   Depositor

   Jiří Hašek

   Article

   Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

   Insert

   ymTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDRF1-GW AKAR3-EV

   Plasmid

   #182533

   Purpose

   Expresses the PKA FRET sensor AKAR3-EV in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

   Insert

   AKAR3-EV
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PMA1

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS406-CMV-lacI-3FLAG

   Plasmid

   #83410

   Purpose

   Expression of lacI in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   lacI repressor
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXP416

   Plasmid

   #26842

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2010 Sep 10. ():.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWS3868

   Plasmid

   #185745

   Purpose

   Sub-array backbone 4/4

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZJOM11

   Plasmid

   #133637

   Purpose

   Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMET17p-CRU313

   Plasmid

   #131163

   Purpose

   For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

   Depositor

   Nils Johnsson

   Article

   Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

   Type

   Empty backbone

   Use

   Tags

   CRU

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

   Plasmid

   #86414

   Purpose

   nuclear split GFP11 reporter without mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-PUS1
   

   Use

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Mutation

   Promoter

   NOP1pr

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-link-yomApple-Kan

    Plasmid

    #44957

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mApple with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomApple
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMJA180

    Plasmid

    #128538

    Purpose

    Expresses E coli Bir A protein

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    E coli BirA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG306GAL-ccdB

    Plasmid

    #14139

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HO-pMET-poly-KanMX4-HO

    Plasmid

    #51665

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection, pMET inducible promoter

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EZ-L580

    Plasmid

    #173023

    Purpose

    Encodes OptoAMP4 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAct::Raf

    Plasmid

    #11341

    Depositor

    Michael Wigler

    Article

    Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

    Insert

    byr-2 (byr2 Fission Yeast)
    

    Use

    Tags

    Gal4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pFA6a-ymTurquoise2-CaURA3

    Plasmid

    #118458

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 426Gal-FUS

    Plasmid

    #29591

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge