7,977 results

1. pKmK.T5

   Plasmid

   #125057

   Purpose

   Storage plasmid for K.marxianus terminator; type 4 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   PGK1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDB4283

   Plasmid

   #98702

   Purpose

   A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   gRNA PCR template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YIp TRP1-KanMx

   Plasmid

   #122923

   Purpose

   TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pDS257

   Plasmid

   #34971

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVW296

   Plasmid

   #139496

   Purpose

   Expression vector for the PP7-mCherry protein in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pADH1 PP7∆FG-mCherry tCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ∆FG

   Promoter

   pADH1

   Available since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPT24

   Plasmid

   #184419

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKmK.P16

   Plasmid

   #125049

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   FBA1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHES836

    Plasmid

    #89195

    Purpose

    Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXNgate22-1HA

    Plasmid

    #105077

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ZJOM157

    Plasmid

    #133640

    Purpose

    Integration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-GFP-Pep12 (PEP12 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG426GPD-Cerulean-ccdB

    Plasmid

    #14420

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIp TRP1-NatMx

    Plasmid

    #122924

    Purpose

    TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCfB6574

    Plasmid

    #106132

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZJOM86

    Plasmid

    #133634

    Purpose

    Integration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ANP1-GFP (ANP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDS244

    Plasmid

    #34982

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Ste11 (STE11 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKT0210

    Plasmid

    #8724

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP A206R
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A,…

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMTU-DO-G418

    Plasmid

    #160007

    Purpose

    Low-copy expression vector for K. marxianus, G418 selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKmK.I3R

    Plasmid

    #125065

    Purpose

    Storage plasmid for K.marxianus right homology arm

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Right homology arm for targeting integration to SWF1/ARO1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge