7,943 results

1. pJB166

   Plasmid

   #86998

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGBT9-Hook3

   Plasmid

   #198525

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook3 (HOOK3 Human)
   

   Use

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. WT (DVD)

   Plasmid

   #184316

   Purpose

   To study dynamics of G-actin using NMR spectroscopy.

   Depositor

   Michael Rosen

   Article

   Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

   Insert

   Act5C (Act5C Fly)
   

   Use

   Tags

   Hexahistidine followed by B-thymosin

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p406 – TDH3-Elo3-GFP11-Ura

   Plasmid

   #177734

   Purpose

   ER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA marker

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   Elo3 (ELO3 Budding Yeast)
   

   Use

   Tags

   GFP11 and HDEL

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pK

   Plasmid

   #64188

   Purpose

   harbours marker gene LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p416-GPD-HA-PPK

   Plasmid

   #183943

   Purpose

   Expresses HA-tagged E. coli PPK from yeast GPD promoter

   Depositor

   Michael Downey

   Insert

   PPK (ppk E. coli)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   WT

   Promoter

   GPD

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYES2.1-TRP1

   Plasmid

   #11007

   Depositor

   Francisco Malagon

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Tags

   V5 and his6

   Expression

   Bacterial and Yeast

   Mutation

   ORF-TRP1 without stop codon

   Promoter

   Available since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG416GPD-ccdB-EGFP

   Plasmid

   #14196

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPS2042

   Plasmid

   #1373

   Depositor

   Pamela Silver

   Article

   Brodsky et al RNA 2000 Dec;6(12):1737-49.

   Insert

   U1A (1-94) (RNU1-4 Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   U1A (1-94)

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pSM1959

    Plasmid

    #41837

    Depositor

    Susan Michaelis

    Article

    Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

    Insert

    SEC63-mRFP
    

    Use

    Tags

    mRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHLM

    Plasmid

    #64168

    Purpose

    harbours marker genes HIS3, LEU2 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS305-mAID-LaM2

    Plasmid

    #198413

    Purpose

    Express mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    mAID-Nb (LaM2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV0584

    Plasmid

    #133471

    Purpose

    Integration into fission yeast genome at ura4 locus with natMX selection cassette

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMAL728

    Plasmid

    #169152

    Purpose

    Encodes OptoQ-AMP6

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG416GAL10-HRV-B14_3C

    Plasmid

    #203466

    Purpose

    Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    HRV-B14 3C protease (HRVBgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEVY-L

    Plasmid

    #51226

    Purpose

    bi-directional expression vector for yeast, constitutively active, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXP318

    Plasmid

    #26837

    Depositor

    Suzanne Sandmeyer, Nancy DaSilva

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKmK.P15

    Plasmid

    #125048

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    TDH3 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS406-PHO5-GFP-hFAPP1 PH domain

    Plasmid

    #58723

    Purpose

    Expresses GFP-Tagged human FAPP1 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only