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Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pYM28-BirA*
Plasmid#160290PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3DepositorInsertBirA*
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0585
Plasmid#133472PurposeIntegration into fission yeast genome at ade6 locus with hphMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p42Nat 1-F GW
Plasmid#58549PurposeMulticopy with clonNat resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-empty chr I
Plasmid#41895DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPDAvailable sinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV0327
Plasmid#133488PurposeExpression of nucleo-cytoplasmic sfGFPDepositorInsertpUra4(AfeI)-p(act1)-sfGFP-terminator(tdh1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p406CYC1
Plasmid#15975DepositorInsertCYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationCYC1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsTagsExpressionYeastMutationPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAS9cd
Plasmid#141252PurposeConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
UseTagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet14
Plasmid#107451PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet14 (met14 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSir1
Plasmid#107447PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSir1 (sir1 Fission Yeast)
UseTagsV5-HisExpressionYeastMutationH523R, A750TPromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet1
Plasmid#107444PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet1
UseTagsV5-HisExpressionYeastMutationPromoterGAL1Available sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G3-Ph
Plasmid#46818PurposeTEF1 and GAL10 promoter controlled expression cassettes with phleomycin resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and GAL10Available sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS_129
Plasmid#160652PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHS3-msGFPx3
Plasmid#105257PurposePlasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.DepositorInsertChs3 portion (CHS3 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-HPH-C4
Plasmid#160343PurposeHigh-copy expression vector for K. marxianus, hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only