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pYES2.1-pomMet8

Plasmid

#107449

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met8

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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p426*-LbSgH

Plasmid

#136992

Purpose

Helper plasmid for LbCpf1 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW (n1)StayGold-T2A-mCherry

Plasmid

#205495

Purpose

Expression of (n1)StayGold-T2A-mCherry in yeast

Depositor

Bas Teusink

Insert

(n1)StayGold-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pLH_Scr15

Plasmid

#100537

Purpose

CEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

Inserts

Phytochrome B (PHYB Mustard Weed)
Phytochrome interacting factor 3 (PIF3 Mustard Weed)

Use

Cre/Lox and Synthetic Biology

Tags

CreC, CreN, and SV40 NLS

Expression

Yeast

Mutation

N-terminal version of PhyB (AA 1-621)/ Mutation o…

Promoter

FBA1 and TDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymYPET-CaURA3

Plasmid

#118460

Purpose

Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Use

Tags

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTH761-CEN-mCherry_v4

Plasmid

#40599

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Use

Tags

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-ymTurquoise2-CaURA3

Plasmid

#118458

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymTurquoise2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pBSWHhc-p53

Plasmid

#133903

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (TP53 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSAC200

Plasmid

#187911

Purpose

psac200-crispey-bar-vector

Depositor

Hunter Fraser

Article

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSAC212

Plasmid

#187912

Purpose

psac212-crispey-bar-ade2KO

Depositor

Hunter Fraser

Article

Chen et al Cell Genom. 2023 Feb 28;3(4):100273. doi

Insert

ade2 KO CRISPEY cassette

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Use

Tags

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pV1090

Plasmid

#111427

Purpose

gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PCUP1-DuDre-Atg8-404

Plasmid

#69201

Purpose

RFP-Atg8 (PAS marker) with improved property

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

DuDre-Atg8

Use

Tags

DsRed Express 2

Expression

Yeast

Mutation

Promoter

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCYC2

Plasmid

#43970

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

CYC1

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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