Addgene

Showing: 1181 - 1200 of 7341 results

Results per page:

Sort results by:

416Gal-FUS-R521C-YFP

Plasmid

#29622

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R521C

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pPICZc-HsACTG1*-thymosinB-8His

Plasmid

#111147

Purpose

Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

actin gamma 1 (ACTG1 Human)

Use

Pichia pastoris integration vector

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

codon-optimised for expression in Pichia pastoris

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
yHS1

Plasmid

#159150

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMW37: pRS426-R2Bm RNA

Plasmid

#209291

Purpose

Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2Bm 5'UTR and 3'UTR

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1 GAPDH

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Plasmid

#140153

Purpose

Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK002-2-pGAP

Plasmid

#84961

Purpose

Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glyceraldehyde-3-phosphate dehydrogenase promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

BsaI site removed (1805 c to g)

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRG216

Plasmid

#64528

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS415_pGal-nCas9(H840A)

Plasmid

#79616

Purpose

Expresses nCas9(H840A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

Expression

Yeast

Mutation

H840A for nickase Cas9

Promoter

pGal1

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZc-HsACTB*-thymosinB-8His

Plasmid

#111146

Purpose

Expresses human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

Promoter

AOX1

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
M3925 trp1::KanMX3 Disruptor Converter

Plasmid

#51681

Purpose

Yeast marker swap plasmid for converting Trp1 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-yomWasabi-CaURA3

Plasmid

#44889

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p42Hyg 1-F GW

Plasmid

#58551

Purpose

Multicopy vector with Hygromycin resistance marker for single Gateway insertion

Depositor

Leonid Kruglyak

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRG203MX

Plasmid

#64521

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-URA-GFP-Ubc9wt

Plasmid

#20368

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p426 46Q GPD

Plasmid

#1182

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 46Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pYZ157

Plasmid

#98413

Purpose

Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT2.2

Plasmid

#11343

Depositor

Guy Caldwell

Type

Empty backbone

Use

Tags

Gal4 Activation Domain

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge