7,941 results

1. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK_P-GAP-ACC1*

   Plasmid

   #126740

   Purpose

   yeast plasmid overexpressing ACC1 from Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acc1*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T3452G

   Promoter

   GAP

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTUP1

   Plasmid

   #104341

   Purpose

   Express TUP1

   Depositor

   Alexander Varshavsky

   Article

   Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

   Insert

   TUP1 (TUP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG423GAL-ccdB-EGFP

   Plasmid

   #14197

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKT127-SEP

   Plasmid

   #41899

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Type

   Empty backbone

   Use

   Tags

   superecliptic GFP (SEP)

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

   Plasmid

   #86418

   Purpose

   ONM/ER split GFP1-10

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP1-10-Scs2TM
   

   Use

   Tags

   GFP1-10

   Expression

   Yeast

   Mutation

   Promoter

   NOP1

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. M4786 KanMX::hisG-URA3-HisG Disruptor Converter

   Plasmid

   #51690

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   hisG-URA3-hisG (URA3 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSP-G2

   Plasmid

   #64737

   Purpose

   Yeast expression plasmid

   Depositor

   Jens Nielsen

   Article

   Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pI1-MTU-DO-HPH

   Plasmid

   #160014

   Purpose

   Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSP-G1

    Plasmid

    #64736

    Purpose

    Yeast expression plasmid

    Depositor

    Jens Nielsen

    Article

    Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. bRA66

    Plasmid

    #100952

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHLU

    Plasmid

    #64167

    Purpose

    harbours marker genes HIS3, LEU2 and URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-SKII-3XHA-HALO-Hygromycin

    Plasmid

    #188929

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Hygromycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Gal1/10p CAS9 Gal1/10p I-SceI

    Plasmid

    #182519

    Purpose

    Galactose-Induced Expression vector for Cas9 and I-SceI.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Inserts

    I-SceI
    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG306GPD-ccdB-DsRed

    Plasmid

    #14356

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

    Plasmid

    #24584

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. BS-Ura3Kl

    Plasmid

    #69195

    Purpose

    complementation of yeast auxotrophic marker URA3(Kluyveromyces lactis)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Kluyveromyces lactis URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJZC588

    Plasmid

    #62315

    Purpose

    sgRNA with 2x MS2 (wt+f6) for yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x MS2 (wt+f6) binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRG714_lexO-AscI_LexA-TF_URA3MX

    Plasmid

    #154822

    Purpose

    Inducible expression of AscI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    AscI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge