7,935 results

1. pCTCON2-Aga1p

   Plasmid

   #158141

   Purpose

   Expresses Aga1p in yeast

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-link-yomWasabi-CaURA3

   Plasmid

   #44889

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mWasabi with CaUra3 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomWasabi
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SJZ7-KAN

   Plasmid

   #228260

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   kan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 S.c.

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SJZ4-NAT

   Plasmid

   #228257

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   nat1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   FBA1 S.c.

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFP5EC4

   Plasmid

   #185880

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pILGFP3AG4ARSd

   Plasmid

   #185876

   Purpose

   A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYX233-b2delta-DHFR

   Plasmid

   #163760

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2delta-DHFR (Dhfr Budding Yeast, Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDRF1-GW ymYPET

   Plasmid

   #118455

   Purpose

   Overexpression of ymYPET

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYES2.1-cerMET8

    Plasmid

    #107439

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET8 (MET8 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

    Plasmid

    #58438

    Purpose

    Expresses LexA-ER-Gal4AD from yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    LexA-ER-Gal4AD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Act1 promoter (-520;1)

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXP422

    Plasmid

    #26845

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDIV537

    Plasmid

    #177704

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LD127

    Plasmid

    #69175

    Purpose

    Integration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg27-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJH2971

    Plasmid

    #100955

    Purpose

    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

    Depositor

    James Haber

    Article

    Ranjith et al Protocol Exchange (2017)

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM151

    Plasmid

    #133643

    Purpose

    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-GFP (COX4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-SKII-URA-GAL1-fSNAP-3xHA

    Plasmid

    #188935

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    URA-GAL1-fSNAP-3xHA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZJOM20

    Plasmid

    #133644

    Purpose

    Integration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-PEX1-2GFP (PEX1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge