7,935 results

1. pCEV-G1-Ph

   Plasmid

   #46814

   Purpose

   TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

   Depositor

   Claudia Vickers, Lars Nielsen

   Article

   Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

   Type

   Empty backbone

   Use

   Tags

   FLAG and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 and PGK1

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yoSuperfolderGFP-Kan

   Plasmid

   #44901

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoSuperfolderGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3HR2

   Plasmid

   #220738

   Purpose

   Component of yeast three hybrid system

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIII/MS2-1

   Plasmid

   #220629

   Purpose

   Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIII/MS2-2

   Plasmid

   #220630

   Purpose

   For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS2-1

   Plasmid

   #220627

   Purpose

   Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS2-2

   Plasmid

   #220628

   Purpose

   Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pG3

   Plasmid

   #162604

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-ABP1-iGFPx6

   Plasmid

   #177099

   Purpose

   Expresses Abp1-iGFPx6 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   ABP1 (ABP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-SKII-3XHA-fSNAP-Hygromycin

    Plasmid

    #188914

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-Hygromycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZJOM119

    Plasmid

    #133661

    Purpose

    Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)
    

    Use

    Tags

    mTagBFP2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. p405TEF1

    Plasmid

    #15968

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    TEF1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS306-pHIS3-2xGFP-tURA3

    Plasmid

    #116936

    Purpose

    Yeast integrative vector for the expression of 2xGFP

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    2xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pE2c

    Plasmid

    #17462

    Purpose

    modified pENTR vector for C-terminal 3x HA tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid

    #165977

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP92

    Plasmid

    #83549

    Purpose

    Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TDH3 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK1

    Plasmid

    #111722

    Purpose

    to express human BLM protein in yeast cells

    Depositor

    Ian Hickson

    Article

    Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    hexahistidine

    Expression

    Yeast

    Mutation

    a C-terminal hexahistidine tag was added, a yeast…

    Promoter

    GAL1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LT2_14_GlyOx

    Plasmid

    #177711

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDRf1-GW

    Plasmid

    #36026

    Depositor

    Wolf Frommer, Dominique Loque

    Article

    Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge