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Plasmid#106153PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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ZJOM57
Plasmid#133656PurposeIntegration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.DepositorInsertp1k-PEX3-DuDre (PEX3 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB6576
Plasmid#106134PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAT222
Plasmid#40942DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP78
Plasmid#78948PurposeSupplemental Figure 3. Integration Site 5.DepositorInsertIntegration Site 5
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
UseTagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…PromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4787
Plasmid#106147PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECitrine-Hygro
Plasmid#44646DepositorInsertyECitrine
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorInsertp53 (Trp53 Mouse)
UseTagsExpressionYeastMutationPromoterT7Available sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-SpHis5
Plasmid#44836PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB
Plasmid#14149DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIM701
Plasmid#74642Purposealias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection markerDepositorInsertymTagBFP
UseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-3FLAG
Plasmid#19337DepositorInsert3FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yAT1.03
Plasmid#132781PurposeFRET biosensor for ATP measurements in budding yeastDepositorInsertyAT1.03 ymTq2-tdTomato
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3FLAG
Plasmid#19338DepositorInsert3FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only