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Addgene
Showing: 5861 - 5880 of 7105 results
  1. pJCKan

    Plasmid
    #185862
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a G418-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; KanMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. pJCHph

    Plasmid
    #185863
    Purpose
    The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker
    Depositor
    Insert
    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pIALD2S

    Plasmid
    #185865
    Purpose
    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae
    Depositor
    Insert
    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pIALD2HMGr

    Plasmid
    #185867
    Purpose
    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae
    Depositor
    Insert
    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pIPKAH

    Plasmid
    #185869
    Purpose
    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)
    Depositor
    Insert
    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pILGFP1DFB

    Plasmid
    #185871
    Purpose
    A parental plasmid for in vivo gene amplification at RPL25 locus
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pILGFP3A5C

    Plasmid
    #185872
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pILGFP3AE4

    Plasmid
    #185873
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pILGFP3AG4

    Plasmid
    #185874
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pILGFP3AG4ARSd

    Plasmid
    #185876
    Purpose
    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 5861 - 5880 of 7105 results