8,173 results

1. pFA6a-link-ymScarletI3-Kan

   Plasmid

   #224077

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selection

   Depositor

   Bas Teusink

   Insert

   ymScarletI3
   

   Tags

   ymScarletI3

   Expression

   Yeast

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PRS303-240xtetO

   Plasmid

   #44754

   Depositor

   Narendra Maheshri

   Article

   Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

   Insert

   240x tetO array and CHRI homology
   

   Expression

   Yeast

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES260-sc.eEF2 K509A

   Plasmid

   #85120

   Purpose

   yeast expression of mutant EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K509A

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKmK.P3

   Plasmid

   #125036

   Purpose

   Storage plasmid for K.marxianus promoter; type 2 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   ENO1 promoter
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. yeast_DualReporter

   Plasmid

   #127546

   Purpose

   A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).

   Depositor

   Aviv Regev

   Article

   de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do

   Inserts

   bla
   mCherry
   URA3 (URA3 Budding Yeast)
   yEVenus
   Nat1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTKM07

   Plasmid

   #233479

   Purpose

   SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKmK.C4

   Plasmid

   #125062

   Purpose

   Storage plasmid for K.marxianus ARS

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   KmARS2
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGR420

   Plasmid

   #213782

   Purpose

   Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank

   Depositor

   Chang Liu

   Insert

   MET15
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZS165 CRISPEY HH-HDV NotI

   Plasmid

   #114451

   Purpose

   Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Type

   Empty backbone

   Use

   CRISPR; Cen/ars

   Expression

   Yeast

   Promoter

   ScGAL7p

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKmK.T3

    Plasmid

    #125055

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KMXK_A3020 terminator
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKmK.I2R

    Plasmid

    #125064

    Purpose

    Storage plasmid for K.marxianus right homology arm

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Right homology arm for targeting integration to chromosome V: 23743..21744
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKmK.I2L

    Plasmid

    #125031

    Purpose

    Storage plasmid for K.marxianus left homology arm; type 1 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Left homology arm for targeting integration to chromosome V: 23743..21744
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG415GPD-ccdB

    Plasmid

    #14146

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

    Plasmid

    #176395

    Purpose

    Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBF3060

    Plasmid

    #26853

    Purpose

    Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Insert

    CreA recombinase
    

    Use

    Cre/Lox

    Expression

    Yeast

    Mutation

    N/A

    Promoter

    GAL1

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKmK.M1

    Plasmid

    #125058

    Purpose

    Storage plasmid for selection marker

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    ScTRP1 marker
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKmK.P19

    Plasmid

    #125052

    Purpose

    Storage plasmid for K.marxianus promoter; type 2 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    LAC4 promoter
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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