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Showing: 541 - 560 of 7341 results

  1. pAWH-largeT

    Plasmid
    #133895
    Purpose
    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Insert
    LargeT
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    T7
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    133895-plasmid-map-sequence-id-266967

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  2. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    104912-plasmid-map-sequence-id-206066

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  11. p406TEF1

    Plasmid
    #15976
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

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  12. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

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  13. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  14. SIVl

    Plasmid
    #81090
    Purpose
    Single Integration Vector LEU - Cloning vector for single integration into the LEU2 locus of S.cerevisiae containing two MCS.
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81090-plasmid-map-sequence-id-164491

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  16. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

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  17. LPH2781 - Ub-K63R

    Plasmid
    #32160
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    K63R
    Promoter
    CUP1
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 541 - 560 of 7341 results