Showing: 5401 - 5420 of 7105 results
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pNA0306
Plasmid#169452PurposepRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmidDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterTEF1pAvailabilityAcademic Institutions and Nonprofits only -
pXZX351
Plasmid#169454PurposepRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassetteDepositorInsertgRNA for MATα
UseTagsExpressionYeastMutationPromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pXZX501
Plasmid#169455PurposepRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassetteDepositorInsertgRNA for MATa
UseTagsExpressionYeastMutationPromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 Empty
Plasmid#169710PurposeYeast two-hybrid vector using a copper inducible CUP1 promoterDepositorArticleTypeEmpty backboneUseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1AvailabilityAcademic Institutions and Nonprofits only -
pHCMM1
Plasmid#167452PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoterDepositorInsertPTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pHCMM2
Plasmid#167453PurposeExpresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoterDepositorInsertPSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
pHCMM3
Plasmid#167454PurposeA control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]DepositorInsertPSEC63_yeGFP_cePUP-2_URA3_TADH1
UseTagsExpressionYeastMutationPromoterPSEC63AvailabilityAcademic Institutions and Nonprofits only -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRTagsExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pGG431
Plasmid#165615PurposeVector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
UseCRISPRTagsSV40 NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-rtetTAK8N, L131L
Plasmid#165968PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGK415-rtetTAK8N, L131L
Plasmid#165969PurposeExpresses rTetTA (K8N, L131L) mutantDepositorInserttetR
UseSynthetic BiologyTagsthree copies of VP16 (VP48)ExpressionYeastMutationPromoterpPGK1AvailabilityAcademic Institutions and Nonprofits only -
pGK415-phlTA2-1E
Plasmid#165970PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailabilityAcademic Institutions and Nonprofits only
Showing: 5401 - 5420 of 7105 results