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pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Expression

Yeast

Mutation

aa 1-215E

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO760

Plasmid

#235759

Purpose

Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38 delta C2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO765

Plasmid

#235762

Purpose

Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO932

Plasmid

#235764

Purpose

Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Tags

3xFLAG

Expression

Yeast

Mutation

aa 268-875

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO503

Plasmid

#235743

Purpose

Protein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genome

Depositor

Roger Williams

Publication

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmTOR

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 4-4609

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO524

Plasmid

#235744

Purpose

Protein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome

Depositor

Roger Williams

Publication

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmLST8

Expression

Yeast

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca+C-Tail

Plasmid

#237526

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca+C-Tail

Expression

Yeast

Mutation

Add C-terminal extension

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca

Plasmid

#237524

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca

Expression

Yeast

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7-AvrPWT4

Plasmid

#237527

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

AvrPwt4

Expression

Yeast

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGBKT7-VirPWT4

Plasmid

#237528

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

VirPwt4

Expression

Yeast

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-NET1

Plasmid

#232893

Purpose

Plasmid expressing Cas9 and gRNA GTGCCACTAATGGATCCATG which targets the NET1 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

NET1 gRNA (NET1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-KAE1

Plasmid

#232891

Purpose

Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

KAE1 gRNA (KAE1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-VRG4

Plasmid

#232899

Purpose

Plasmid expressing Cas9 and gRNA TCGCATAGCCCAGTATACGT which targets the VRG4 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

VRG4 gRNA (VRG4 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pML107-SAM50

Plasmid

#232895

Purpose

Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

Depositor

Jolanda Van Leeuwen

Publication

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

SAM50 gRNA (SAM50 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

snR52

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge