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pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO934

Plasmid

#235766

Purpose

Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta CBR1

Tags

3xFLAG

Expression

Yeast

Mutation

aa 31-55 deleted

Promoter

ScVPS34 promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A, D731N

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Expression

Yeast

Mutation

aa 268-875

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Tags

ZZ-3xTEV

Expression

Yeast

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Expression

Yeast

Mutation

aa 1-215E

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO760

Plasmid

#235759

Purpose

Expression of ScVPS38 delta C2 (1-154)-x3FLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38 delta C2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO761

Plasmid

#235760

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-x3FLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

3xFLAG

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO765

Plasmid

#235762

Purpose

Expression of ScVPS38 delta C2 (1-154) -EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Mutation

aa 1-154 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO766

Plasmid

#235763

Purpose

Expression of ScVPS38 delta BARA2 (320-440)-EGFP under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VSP38 delta BARA2

Tags

EGFP

Expression

Yeast

Mutation

aa 320-440 deleted

Promoter

ScVPS38 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO932

Plasmid

#235764

Purpose

Expression of ScVPS34 HELCAT (268-875)-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Tags

3xFLAG

Expression

Yeast

Mutation

aa 268-875

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO933

Plasmid

#235765

Purpose

Expression of ScVPS34 delta C154-V202-3xFLAG under its own promoter

Depositor

Roger Williams

Publication

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta C154-V202

Tags

3xFLAG

Expression

Yeast

Mutation

aa 154-202 deleted

Promoter

ScVPS34 promoter

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO503

Plasmid

#235743

Purpose

Protein expression of KmTor by integrating ZZ-KmTOR into the K. marxianus genome

Depositor

Roger Williams

Publication

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmTOR

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 4-4609

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO524

Plasmid

#235744

Purpose

Protein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome

Depositor

Roger Williams

Publication

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmLST8

Expression

Yeast

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7-RWT4Ca_Reg1

Plasmid

#237525

Purpose

Yeast two-hybrid

Depositor

Gitta Coaker

Publication

Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

Insert

Rwt4Ca_Reg1

Expression

Yeast

Mutation

Removed binding region 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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