Skip to main content
Addgene
Showing: 4841 - 4860 of 7342 results

  2. pGADT7-γ1

    Plasmid
    #197078
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-1 γ1 (Ap1g1 Mouse)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197078-plasmid-map-sequence-id-387046

    Enlarge

  3. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-4 ε (AP4E1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197261-plasmid-map-sequence-id-387043

    Enlarge

  10. pCAS-mak10 gRNA-MAK10Δ

    Plasmid
    #160384
    Purpose
    For CRISPR-mediated deletin og MAK10 in yeast
    Depositor
    John McCusker
    Insert
    MAK10
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    160384-plasmid-map-sequence-id-384173

    Enlarge

  11. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

    Enlarge

  20. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

    Enlarge

Showing: 4841 - 4860 of 7342 results