Purpose
Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
Inserts
tyrosinase cytosolic tail
AP-3 σ3A
Use
Tags
GAL4-DNA binding domain fragment, HA tag, and nuc…
Expression
Yeast
Mutation
silent substitution in codon 2 of tyrosinase tail…
Available since
March 28, 2023
Availability
Academic Institutions and Nonprofits only