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Plasmid#193322PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M2C5
Plasmid#188606PurposeMinimal Conjugative Plasmid 2DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M1C1
Plasmid#188609PurposeMinimal Conjugative Plasmid 1DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C16
Plasmid#188610PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
M4C19
Plasmid#188611PurposeMinimal Conjugative Plasmid 4DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-Cup1p-FusionRed
Plasmid#188392PurposeCu2+-dependent expression of red fluorescent protein in yeast cellsDepositorInsertFusionRed
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ4
Plasmid#198176PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-4 μ4
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymTurquoise2
Plasmid#193958PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymTurquoise2 in budding yeastDepositorInsertymTurquoise2
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA2-GAE
Plasmid#199175PurposeExpression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA2-GAE domain (GGA2 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationContains Lys 545 Gln substitutionPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-γ1 ear
Plasmid#199177PurposeExpression of GAL4 DNA-binding domain (BD)-AP-1 γ1 ear domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 γ1 ear domain (Ap1g1 Mouse)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA3-GAE
Plasmid#199176PurposeExpression of GAL4 DNA-binding domain (BD)-GGA3-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA3-GAE domain (GGA3 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-γ2 ear
Plasmid#199178PurposeExpression of GAL4 DNA-binding domain (BD)-AP-1 γ2 ear domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-1 γ2 ear domain (AP1G2 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-GGA1-GAE
Plasmid#199174PurposeExpression of GAL4 DNA-binding domain (BD)-GGA1-GAE domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertGGA1-GAE domain (GGA1 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ATG9A-N
Plasmid#198530PurposeExpression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)DepositorInsertATG9A N-terminal cytosolic tail (residues 1-66) (ATG9A Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FTS
Plasmid#198526PurposeExpression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)DepositorInsertFTS (AKTIP Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-FHIP-L
Plasmid#198528PurposeExpression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)DepositorInsertFHIP-L (FHIP1A Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook1
Plasmid#198523PurposeExpression of GAL4 DNA-binding domain (BD)-Hook1 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook1 (HOOK1 Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 620 to eliminate int…PromoterADH1Available sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-δ
Plasmid#197259PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-3 δ (AP3D1 Human)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationVal 549 Ile substitution; silent substitutions in…PromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-ubiquitin
Plasmid#196999PurposeExpression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)DepositorInsertubiquitin (UBC Human)
UseTagsGAL4-DNA binding domain fragmentExpressionYeastMutationPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-Rabex-5
Plasmid#197043PurposeExpression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertRabex-5 (RABGEF1 Bovine)
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only