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Plasmid#212682Purposeintegration vector for single cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCfB13015
Plasmid#212686Purposeintegration vector for single cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB13016
Plasmid#212687Purposeintegration vector for single cassette with integration site E3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12713
Plasmid#212684Purposeintegration vector for single cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12716
Plasmid#212690Purposeintegration vector for three gene cassette with integration site E4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB12714
Plasmid#212688Purposeintegration vector for three gene cassette with integration site E2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆RPromoterpATG8Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-4V5-Pho8-Met
Plasmid#207029PurposeExpression of 4V5-Pho8 under CPU1 promoter.DepositorInsertPho8
UseTags4xV5ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp-PCUP1-4myc-Pho8
Plasmid#207028PurposeExpression of 4Myc-Pho8 under CPU1 promoter.DepositorInsertPho8
UseTags4xMycExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-Vac8-GFP-Ura
Plasmid#207027PurposeExpression of Vac8-GFP under CPU1 promoter.DepositorInsertVac8
UseTagsGFPExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Vma13-Ura
Plasmid#207026PurposeExpression of GFP-Vma13 under CPU1 promoter.DepositorInsertVma13 ORF
UseTagsGFPExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆GR-MET15
Plasmid#207033PurposeExpression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆GRPromoterpATG8Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-mRuby3-Pho8-406
Plasmid#207043PurposeExpression of mRuby3-Pho8 under CPU1 promoter.DepositorInsertPHO8
UseTagsmRuby3ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXOOY
Plasmid#196449PurposeA dual vector for expression of membrane proteins in S. cerevisiae and X. laevisDepositorTypeEmpty backboneUseExpression in xenopus laevisTagsExpressionYeastMutationPromoteryeast CYC-GAL and T7 promoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only