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pLK89 - Edit-directing plasmid cloning insert

Plasmid

#98846

Purpose

Contains the insert used for step 2 of edit-directed plasmid cloning

Depositor

Leonid Kruglyak

Insert

KanR

Use

CRISPR

Tags

Expression

Yeast

Mutation

Changed sequence to remove a MluI cut site

Promoter

Availability

Academic Institutions and Nonprofits only

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pIW447-CRISPR XYL2

Plasmid

#98906

Purpose

K. marxianus CRISPR Plasmid for XYL2 editing

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pIW601-KmCRISPR

Plasmid

#98907

Purpose

K. marxianus CRISPR Plasmid for sgRNA cloning

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pMVS102_P3-GFP-NATMX6

Plasmid

#99048

Purpose

EGFP reporter with six binding sites for the Zif268 DNA binding domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

McIsaac 2014 P3 promoter
eGFP
clonNAT resistance

Use

Tags

Expression

Yeast

Mutation

GAL1 promoter with 4 GAL4 sites removed and repla…

Promoter

McIsaac 2014 P3 promoter

Availability

Academic Institutions and Nonprofits only

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pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

Plasmid

#99049

Purpose

Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Depositor

Barak Cohen

Article

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

ACT1 promoter (ACT1 Budding Yeast)
mCherry
Zif268 DNA binding Domain
Estrogen response domain (ESR1 Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pRS303-PADH1-AFB2

Plasmid

#99530

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Availability

Academic Institutions and Nonprofits only

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YIp204-PADH1-AFB2

Plasmid

#99531

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Availability

Academic Institutions and Nonprofits only

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YIp204-PADH1-atTIR1-9myc

Plasmid

#99532

Purpose

S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

TIR1 (TIR1 Mustard Weed)

Use

Tags

9xMyc

Expression

Yeast

Mutation

Promoter

ADH1

Availability

Academic Institutions and Nonprofits only

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YEp181-CUP1-His-Smt3

Plasmid

#99536

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); LEU2 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp112-CUP1-His-Smt3

Plasmid

#99537

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp195-CUP1-His-Smt3

Plasmid

#99538

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp181-CUP1-His-Ubi

Plasmid

#99539

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
YEp195-CUP1-His-Ubi

Plasmid

#99540

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Use

Tags

7xHis

Expression

Yeast

Mutation

Promoter

CUP1

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-P30-His-POL30

Plasmid

#99542

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K127R)

Plasmid

#99543

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K164R)

Plasmid

#99544

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-P30-His-pol30(K127/164R)

Plasmid

#99545

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Availability

Academic Institutions and Nonprofits only

Enlarge