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Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P), DHFR (P66L)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL3C
Plasmid#218275PurposeIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM014_P2_pGPM1
Plasmid#216439PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter gpm1
UseSynthetic BiologyTagsExpressionYeastMutationRemoval of any BsaI // BsmBI restriction sitePromoterAvailable sinceMay 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH3128
Plasmid#210165PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1 (ADE2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRH2941
Plasmid#210143PurposeProtein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1DepositorInsertYIp ADE2-LEU2 pTDH3::ARO7-GFP::tADH1 (ARO7 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Beta-Amyloid
Plasmid#181730PurposeGalactose inducible expression of Prepro-3x-Beta-AmyloidDepositorInsertPrepro-3x-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Adan
Plasmid#181733PurposeGalactose inducible expression of Prepro-3x-AdanDepositorInsertPrepro-3x-Adan
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-M337V
Plasmid#181721PurposeGalactose inducible expression of TDP-43-M337VDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationM337VPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-N267S
Plasmid#181720PurposeGalactose inducible expression of TDP-43-N267SDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationN267SPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-TDP-43-Q331K
Plasmid#181719PurposeGalactose inducible expression of TDP-43-Q331KDepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationQ331KPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-R521C
Plasmid#181718PurposeGalactose inducible expression of FUS-R521CDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationR521CPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-P431L
Plasmid#181717PurposeGalactose inducible expression of FUS-P431LDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationP431LPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Kar2-Beta-Amyloid
Plasmid#181707PurposeGalactose inducible expression of Kar2-Beta-AmyloidDepositorInsertKar2-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-RNQ1
Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4A8_M59I_T94M_BC
Plasmid#217833Purpose4A8 variant in barcoded, yeast surface display plasmidDepositorInsert4A8 variant Fab
UseTagsExpressionYeastMutationVH: M59I, VL: T94MPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD_lambda_mRFP
Plasmid#217831PurposeYeast surface display Fab vector with lambda light chain and mRFPDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD_kappa_mRFP
Plasmid#217830PurposeYeast surface display Fab vector with kappa light chain and mRFPDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Osh1-406
Plasmid#207007PurposeIntegration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.DepositorInsertOSH1
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only