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Plasmid#222494PurposeCo-expression of two genes in yeast with V5 and HA tags and THR1 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only
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pESC-THR
Plasmid#222479PurposeCo-expression of two genes in yeast with FLAG and MYC tags and THR1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE
Plasmid#222480PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ADE2 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ARG
Plasmid#222481PurposeCo-expression of two genes in yeast with FLAG and MYC tags and ARG1 selectionDepositorTypeEmpty backboneUseTagsFLAG and MycExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3HR2
Plasmid#220738PurposeComponent of yeast three hybrid systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIII/MS2-1
Plasmid#220629PurposeYeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIII/MS2-2
Plasmid#220630PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTr2GCsgScLEU2
Plasmid#224868PurposeDisruption of S. cerevisiae type LEU2 geneDepositorInsertpYAMTr2GC having the guide sequence ScLEU2 (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-134-YFP
Plasmid#224882PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 10 amino acids of the proline-rich region (125-134) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-134 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-134PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP135-170-YFP
Plasmid#224878PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 36 amino acids of the proline-rich region (135-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP135-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP135-170PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-160-YFP
Plasmid#224886PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the middle 20 amino acids of the proline-rich region (141-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterGPDAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP125-160-YFP
Plasmid#224883PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.DepositorInsertHtt103QdeltaP125-160 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP125-160PromoterCUP1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 GAL1pro-YFP
Plasmid#224875PurposeLow copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)DepositorTypeEmpty backboneUseTagsYFPExpressionYeastMutationPromoterGAL1Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-M-PRR-Htt97QP4-YFP
Plasmid#224885PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct with the last 46 amino acids of the proline-rich region at the N-terminus, 4 prolines left at the C-terminus using copper.DepositorInsertM-PRR-Htt97QP4 (HTT Human)
UseTagsYFPExpressionYeastMutationN-PRRPromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP141-170-YFP
Plasmid#224880PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.DepositorInsertHtt103QdeltaP141-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdeltaP141-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS416 CUP1pro-Htt103QdeltaP161-170-YFP
Plasmid#224876PurposeLow copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.DepositorInsertHTT103QdeltaP161-170 (HTT Human)
UseTagsYFPExpressionYeastMutationdelta161-170PromoterCUP1Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1426
Plasmid#218622PurposeExpress tNCS to the peroxisome for toxicity assayDepositorInserttNCS
UseTagsYFP-ePTS1ExpressionYeastMutation106-588PromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1462
Plasmid#218623PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with low expression TEV proteaseDepositorInsertYFP
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1463
Plasmid#218624PurposeExpress mKate-TEV_cutSite_YFP to the peroxisome with high expression TEV proteaseDepositorInsertYFP
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only