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pRS406 ATG9-GFP S122D

Plasmid

#114667

Purpose

Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Use

Tags

GFP

Expression

Yeast

Mutation

changed serine 122 to aspartic acid

Promoter

ATG9

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pXP721

Plasmid

#46055

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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LHP1263 - HA-VPS9

Plasmid

#32431

Depositor

Linda Hicke

Article

Shih et al EMBO J. 2003 Mar 17;22(6):1273-81.

Insert

VPS9 (VPS9 Budding Yeast)

Use

Tags

3xHA

Expression

Yeast

Mutation

Promoter

Lac

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
CIPK2-upGFP

Plasmid

#117863

Purpose

protein expression

Depositor

Geoffrey Chang

Insert

CIPK2-upGFP (CIPK2 Mustard Weed)

Use

Tags

eGFP

Expression

Yeast

Mutation

Promoter

AOX

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pETCON-DnvLB16 M40R;W41H;H42S

Plasmid

#45125

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

DnvLB16 M40R;W41H;H42S

Use

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Mutation

M40R;W41H;H42S to DnvLB16

Promoter

GAL

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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Ube2u

Plasmid

#31428

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2U Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJW1675

Plasmid

#112049

Purpose

CEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

TSC13

Use

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ166

Plasmid

#98419

Purpose

Pombe Expression Vector - nmt41 promoter with Sp.his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ167

Plasmid

#98420

Purpose

Pombe Expression Vector - nmt81 promoter with Sp.his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ175

Plasmid

#98422

Purpose

Pombe Expression Vector - nmt41 promoter with Sp.lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ176

Plasmid

#98423

Purpose

Pombe Expression Vector - nmt81 promoter with Sp.his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ182

Plasmid

#98424

Purpose

Pombe Expression Vector - nmt1 promoter with empty BsaI-pad - no marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB-TAP

Plasmid

#14273

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

TAP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pTK023

Plasmid

#44860

Depositor

Michael Grunstein

Article

Kitada et al Genes Dev. 2012 Nov 1;26(21):2443-55. do

Insert

ADH4-PURA3-URA3-G8-yEGFP1-CLN2PD-NLSSV40-TURA3-TG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pRS414 GPD-Sis1deltaG/F

Plasmid

#18688

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 deltaG/F

Use

Tags

Expression

Yeast

Mutation

Sis1 with the G/F region deleted

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-LFA

Plasmid

#20051

Depositor

Harmit Malik

Article

Elde et al Nature. 2008 Nov 30. ():.

Insert

Protein kinase R (EIF2AK2 Human)

Use

Tags

Expression

Yeast

Mutation

S492A

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCETT2

Plasmid

#19784

Depositor

Jumi Shin

Article

Chen et al Anal Biochem. 2008 Nov 15. 382(2):101-6.

Type

Empty backbone

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pGALSc104(G217S/T499I)

Plasmid

#1151

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G217S/T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pGALSc104(T499I)

Plasmid

#1150

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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