Showing: 3501 - 3520 of 7103 results
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Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
UseTagsExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3AvailabilityAcademic Institutions and Nonprofits only -
pTH862-CEN-slowmaxRLuc/minCFLuc
Plasmid#115371PurposeExpresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferaseDepositorInsertsFirefly Luciferase (codon minimised)
Gcn4 5'-UTR + Renilla luciferase (codon optimised)
UseTagsExpressionYeastMutationAll codons have been changed for the most favoura…PromoterADH1 and TDH3AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo
Plasmid#115421PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Erg11-iGFPx3
Plasmid#115422PurposeExpress Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertErg11-iGFPx3
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Sec31-yoHalo
Plasmid#115423PurposeExpress Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec31-yoHalo
UseTagsyoHaloTagExpressionYeastMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
pSec7-iGFPx6
Plasmid#115424PurposeExpress Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-iGFPx6
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
pSec7-Halox2
Plasmid#115425PurposeExpress Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-Halox2
UseTagsHaloTagExpressionYeastMutationPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
YIplac211-vps10-104
Plasmid#115426PurposeGenerate the vps10-104 allele via pop-in pop-out mutagenesisDepositorInsertvps10-104
UseTagsExpressionYeastMutationdomain 1 deletionPromoterEndogenousAvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#115428PurposeExpresses a non-glycosylated regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1AvailabilityAcademic Institutions and Nonprofits only -
pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)
Plasmid#115429PurposeSelection for in-frame fusion expression constructsDepositorTypeEmpty backboneUseTagsT2A-SpHIS5 and λNExpressionYeastMutationPromoterPGK1AvailabilityAcademic Institutions and Nonprofits only -
pRPL28 CaURA3 (pNTI592)
Plasmid#115431PurposeURA3-marked RPS28DepositorInsertsUseTagsExpressionYeastMutationKpnI site in intronPromoterAshbya gossypii and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
Plasmid#115432PurposeVector for expressing RPL28 with mutagenized exon2DepositorInsertsUseTagsExpressionYeastMutationexon 2 deleted, with MfeI / NheI cloning sitePromoterAshbya gospii TEF1 and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailabilityAcademic Institutions and Nonprofits only -
pCHCS163.2-2.2
Plasmid#115527PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 3501 - 3520 of 7103 results