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Addgene
Showing: 3441 - 3460 of 7103 results

  1. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  2. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  3. pCAS9id_TRP

    Plasmid
    #141254
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Availability
    Academic Institutions and Nonprofits only
    141254-plasmid-map-sequence-id-308894

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  9. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

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  11. ADH1pro_CBF1_SIR4

    Plasmid
    #138616
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    CBF1 (CBF1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138616-plasmid-map-sequence-id-275117

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  12. ADH1pro_TYE7_SIR4

    Plasmid
    #138617
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    TYE7
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138617-plasmid-map-sequence-id-274925

    Enlarge

  13. ADH1pro_GCR1_SIR4

    Plasmid
    #138618
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR1 (GCR1 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138618-plasmid-map-sequence-id-274926

    Enlarge

  14. ADH1pro_GCR2_SIR4

    Plasmid
    #138619
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCR2 (GCR2 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138619-plasmid-map-sequence-id-274988

    Enlarge

  15. ADH1pro_GCN4_SIR4

    Plasmid
    #138620
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCN4 (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138620-plasmid-map-sequence-id-334951

    Enlarge

  16. ADH1pro_GAL4_SIR4

    Plasmid
    #138621
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GAL4 (GAL4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Availability
    Academic Institutions and Nonprofits only
    138621-plasmid-map-sequence-id-334953

    Enlarge

  17. TY5_hisAI_2micron

    Plasmid
    #138622
    Purpose
    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment
    Depositor
    Rob Mitra
    Insert
    TY5
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Gal1_10
    Availability
    Academic Institutions and Nonprofits only
    138622-plasmid-map-sequence-id-334952

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  18. TEF2pro_mCherry_YFP

    Plasmid
    #138623
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138623-plasmid-map-sequence-id-274912

    Enlarge

  19. TEF1pro_mCherry_YFP

    Plasmid
    #138624
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Rob Mitra
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    138624-plasmid-map-sequence-id-274931

    Enlarge

Showing: 3441 - 3460 of 7103 results