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Plasmid#64521PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only
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pIIIA/MS2-2
Plasmid#220632PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterINO4Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXP218
Plasmid#26831DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-FUS-G156E
Plasmid#181716PurposeGalactose inducible expression of FUS-G156EDepositorInsertFUS (FUS Human)
UseTagsExpressionYeastMutationG156EPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZS165 CRISPEY HH-HDV NotI
Plasmid#114451PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsTagsExpressionYeastMutationPromoterScGAL7pAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0757
Plasmid#133509PurposeExpression of 3GFP tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastMutationPromoterAOX1Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
UseTagsmCherryExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ156
Plasmid#98412PurposePombe Expression Vector - nmt41 promoter with Sp.ura4 markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_101
Plasmid#160541PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-Kan
Plasmid#44903PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selectionDepositorInsertyomCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS416-yZ3EV-Z3pr-yEGFP (RB3579)
Plasmid#69100PurposeURA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFPDepositorInsertsyZ3EV cassette
Z3 promoter
yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G1-Km
Plasmid#46813PurposeTEF1 and PGK1 promoter controlled expression cassettes with G418 resistance for yeast transformationDepositorTypeEmpty backboneUseTagsFLAG and c-mycExpressionYeastMutationPromoterTEF1 and PGK1Available sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only