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Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0587
Plasmid#133474PurposeIntegration into fission yeast genome at his5 locus with bleMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLexA::nud-1
Plasmid#11339DepositorInsertnud-1 (nud-1 Nematode)
UseTagsLexA DNA Binding DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLexA::lis-1
Plasmid#11338DepositorInsertlis-1 (lis-1 Nematode)
UseTagsLexA DNA Binding DomainExpressionYeastMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
UseTags3X-FLAG/8X-Gly linkerExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH730-staRLuc
Plasmid#38226DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationPromoterTDH3 (=GPD)Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNC952
Plasmid#20401DepositorInsertCFP-reporter
UseTagsExpressionYeastMutationX=UbiM (see schematic) linker=deltakPromoterAvailable sinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
GTL-y
Plasmid#81100PurposeGene Tagging vector LEU/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046256
Plasmid#153937PurposeYeast expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;MBPExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046627
Plasmid#153916PurposeYeast expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCGLex
Plasmid#17264DepositorTypeEmpty backboneUseTagsLexAExpressionYeastMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
AtAUX1
Plasmid#117087Purposeprotein expression in yeastDepositorInsertAtAUX1
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
AtPIN1
Plasmid#117088Purposeprotein expression in yeastDepositorInsertAtPIN1
UseTagsHisExpressionYeastMutationPromoterAOXAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046248
Plasmid#153865PurposeYeast expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS426-ACT_unstable
Plasmid#127608Purposehigh-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3DepositorInsertUnstable ACT1 intron (WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS426-ECM_stable
Plasmid#127606Purposehigh-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3DepositorInsertStable ECM33 intron (WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GTU-r
Plasmid#81096PurposeGene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCherry
UseCre/LoxTagsmCherryExpressionYeastMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSec7-iGFPx6
Plasmid#115424PurposeExpress Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertSec7-iGFPx6
UseTagsiGFPExpressionYeastMutationPromoterEndogenousAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-ccdB-TAP
Plasmid#14264DepositorTypeEmpty backboneUseGateway destinationTagsTAPExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only