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Plasmid#105256PurposePlasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.DepositorInsertChc1 portion (CHC1 Budding Yeast)
UseTagsmsGFPx3ExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK096
Plasmid#65203PurposePre-Assembled Ura3 integration vector to be used in the Dueber YTK system.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCT-L7.5.1
Plasmid#42900DepositorInsertL7.5.1
UseTagsHA and c-mycExpressionYeastMutationPromoterGal1-10Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046464
Plasmid#148970PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCLASP_Crz1(19A)_CLASP_RGS2membrane
Plasmid#133085PurposePlasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.DepositorInsertCrz1*-CLASP
UseTagsExpressionBacterial and YeastMutationPromoterpADH1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-KanMX6-Purg1-3FLAG
Plasmid#19354DepositorInserturg1 promoter
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJZC595
Plasmid#62322PurposeExpresses MCP-VP64 and dCas9 in Yeast cellsDepositorInsertsMCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationHas nuclease-inactivating mutations in the RuvC1 …PromoterTdh3 and pAdhAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046337
Plasmid#145566PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDR-GW FLIPglu-2µΔ13V
Plasmid#28000DepositorInsertFLIPglu-2µΔ13V
UseTagseCFP and eYFPExpressionYeastMutationH152APromoterAvailable sinceJune 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVD_85
Plasmid#149604Purpose(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylRDepositorInsertXylR
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FTKnatAX
Plasmid#73899Purposefission yeast drug counter-selectable selection markerDepositorInsertTK-nat
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBES2
Plasmid#83800PurposeUseful for tagging yeast proteins with N-terminal Venus tag; G418 selectionDepositorInsertVenus
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
LD127
Plasmid#69175PurposeIntegration of GFP tag at Atg27 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg27-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1
Plasmid#101084PurposeGA biosensor expression plasmidDepositorInsertGPS1
UseTags6HIS and cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHES854
Plasmid#89197PurposeExpresses mKate2 under control of TDH3 constitutive promoter.DepositorInsertpTDH3-mKate2
UseTagsExpressionYeastMutationPromoterTDH3Available sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSD02
Plasmid#16009DepositorInsertACT1pr-DsRed.T1.N1
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pJW1510
Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
UseTagsExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
p404GALS
Plasmid#17435DepositorInsertGALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGALS promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only