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pXP414

Plasmid

#26841

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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cyterm-ymScarletI pDRF1-GW

Plasmid

#168082

Purpose

Expresses Cyterm-ymScarletI to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

cyterm-ymScarletI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-iYFPx6

Plasmid

#105272

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Use

Tags

iYFPx6

Expression

Yeast

Mutation

Promoter

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0750

Plasmid

#133484

Purpose

Expression in fission yeast from promoter purg1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAG306GPD-ccdB-HA

Plasmid

#14236

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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HsSPHK2

Plasmid

#118092

Purpose

Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

Depositor

Kevin Lynch

Article

Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

Insert

Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1, 10

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pRG223MX

Plasmid

#64523

Purpose

Episomal E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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POM43-mCherry

Plasmid

#69857

Purpose

N-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCherry minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pKT0103

Plasmid

#8718

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yEVenus

Use

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F46L, S65G, V68L, S72A, M153T, V163A, …

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-PATagRFP-KanMX6

Plasmid

#87027

Purpose

Tag S. pombe genes in C terminal with PATagRFP

Depositor

Julien Berro

Insert

PATagRFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJKW1813

Plasmid

#174003

Purpose

Expresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP90G4
ATR1 (ATR1 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSNAPf-AP

Plasmid

#61830

Purpose

Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

SNAP-AP

Use

Pcr template

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only
BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Use

Tags

Expression

Yeast

Mutation

Promoter

Met3

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GAL-ccdB-EGFP

Plasmid

#14191

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-170nΔ13V

Plasmid

#27999

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-170nΔ13V

Use

Tags

eCFP and eYFP

Expression

Yeast

Mutation

WT binding protein (no F16A)

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pTH760-CEN-mCherry_v3

Plasmid

#40598

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences

Use

Tags

Expression

Yeast

Mutation

mCherry ORF partially codon optimised for yeast e…

Promoter

GPD (TDH3)

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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