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Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1
Plasmid#58434PurposeInduces Citrine expression from a promoter containing 4 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorInsertyeast enhanced GFP
UseTagsExpressionYeastMutationPromoterpCYC1Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
UseTagsBFP, flagExpressionYeastMutationPromoterTDH3Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR
Plasmid#24585DepositorInsertpADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR
UseTagsExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…PromoterAvailable sinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSH62-EBD
Plasmid#49455Purposeyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinaseDepositorInsertCre-EBD fusion
UseCre/LoxTagsExpressionYeastMutationPromoterGal1 promoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG713_lexO-HO_LexA-TF_URA3MX
Plasmid#154821PurposeInducible expression of HO endonuclease in S. cerevisiaeDepositorInsertsUseTagsFLAGExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDS2142
Plasmid#184273PurposeTet-off genome insertion systemDepositorInsertCatTA
UseTagsExpressionYeastMutationPromoterTDH3Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAHYB-GFP
Plasmid#85074PurposeExpression of GFP in Pichia pastorisDepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAOX1Available sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKanB alpha
Plasmid#223524PurposeUsed for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAOX1Available sinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCT2
Plasmid#130970PurposeHyPr plasmid with natMX markerDepositorInsertempty backbone
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMK34
Plasmid#130971PurposeHyPr plasmid with hphMX markerDepositorInsertempty backbone
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJB1561
Plasmid#218626PurposeExpress geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimerDepositorInsertGES
UseTagsePTS1ExpressionYeastMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only