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pCE-BiFC-VN173

Plasmid

#22019

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VN173

Use

Tags

Myc

Expression

Worm

Mutation

N/A

Promoter

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Use

Tags

mNeonGreen

Expression

Worm

Mutation

Promoter

myo-2

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHygR2

Plasmid

#200071

Purpose

hygR selection nls-mScarlet marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBN396

Plasmid

#109326

Purpose

Expresses FLP enzyme and fluorescent mNeonGreen in the C. elegans germ line

Depositor

Peter Askjaer

Article

Macias-Leon et al MicroPubl Biol. 2018 Mar 19;2018:10.1791

Insert

mex-5 (mex-5 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

mex-5

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJR014

Plasmid

#198813

Purpose

Natural light-gated anion channels. In vivo inhibition of neurons using blue light.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::GtACR2::SL2::GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGC185

Plasmid

#19624

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)

Use

Flp/frt

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pSR40

Plasmid

#69257

Purpose

plin-31::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Tags

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

lin-31

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJL44 - Phsp16.48::MosTase::glh-2utr

Plasmid

#19333

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Phsp-16::Mos1 transposase

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pJW2086

Plasmid

#154335

Purpose

Crispr repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::GFP::AID*::3xFLAG::10aa linker

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSM08

Plasmid

#139042

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mscarlet (includes STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM235 - [Pmlc-1 | mcherry | cbr-tbb-2 UTR]

Plasmid

#159900

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | mcherry | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Pmlc-1

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

Plasmid

#154824

Purpose

(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

Peft-3::wrmScarlet::tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

eef-1A.1 (eft-3)

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1655

Plasmid

#51481

Purpose

miniMos transposable element with HygroR, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pJM483

Plasmid

#52987

Purpose

A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Use

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pLT61

Plasmid

#1690

Depositor

Andrew Fire

Type

Empty backbone

Use

RNAi

Tags

T7p, unc224616, unc2218148, unc2218082, unc223827…

Expression

Worm

Mutation

Promoter

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

Plasmid

#71719

Purpose

Expressing a GFP-tagged degron in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

auxin-responsive protein IAA17 (AXR3 Mustard Weed)

Use

Tags

EmGFP

Expression

Worm

Mutation

minimal degron sequence (71-114aa) with start cod…

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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