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pHA853 - mec-4p::Fynempty

Plasmid

#139210

Purpose

Control vector for Fyn kinase expression in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA847 - mec-4p::hrp-1mScarlet

Plasmid

#139201

Purpose

Expresses mScarlet tagged hrp-1 in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA844 - hrp-1p::hrp-1mScarlet

Plasmid

#139204

Purpose

Expresses mScarlet tagged hrp-1

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA850 - osm-10p::FynY531F

Plasmid

#139207

Purpose

Express activated Fyn kinase in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA851 - osm-10p::Fynempty

Plasmid

#139208

Purpose

Control vector for Fyn kinase expression in osm-10 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

deletion and frame shift to prevent Fyn expression

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHA852 - mec-4p::FynY531F

Plasmid

#139209

Purpose

Expresses activated Fyn kinase in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

Fyn (FYN Human)

Use

Tags

Expression

Worm

Mutation

Y531F

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Plasmid

#159838

Purpose

Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Plasmid

#159817

Purpose

Positive control for germline GFP expression from simple array in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP5605(cbr-unc-119)[Psmu-1 |smu-1 genomic | GFP-smu-1UTR]

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Zachary Knight, Jennifer Garrison

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Use

Tags

NLS

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1816

Plasmid

#154320

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1884

Plasmid

#154332

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1948

Plasmid

#154340

Purpose

Enhancer reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge