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pNM3814

Plasmid

#154122

Purpose

mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::rtetR-L-QFAD::act-4 '3

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pNM3724

Plasmid

#154112

Purpose

tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

tetO 7X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBN154

Plasmid

#86718

Purpose

FLP/Frt dual color reporter for C. elegans

Depositor

Peter Askjaer

Article

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Phsp16.41::FRT::mCh::his-58::FRT::gfp::his-58

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHygG2

Plasmid

#200066

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGLOW30

Plasmid

#172697

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mScarlet

Use

Tags

mScarlet

Expression

Worm

Mutation

Promoter

myo-2

Available since

Sept. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJR025

Plasmid

#198832

Purpose

Actin filament and cell cortex localization.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::act2-venus::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Worm and Yeast

Mutation

Promoter

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJR022

Plasmid

#198820

Purpose

C. elegans optimized version of mScarlet

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::wrmScarlet::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSH83

Plasmid

#212989

Purpose

expresses miniSOG in C. elegans

Depositor

David M. Miller

Article

Cuentas-Condori et al Elife. 2019 Oct 4;8. pii: 47918. doi: 10

Insert

flp-13 promoter (flp-13 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

flp-13

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJL046

Plasmid

#198807

Purpose

In vivo neuronal inhibition through histamine-gated chloride channel. Neurons expressing HisCl1 transgene are inhibited after addition of histamine.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::HisCl1-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM246 - MCS mosTI unc-119 target

Plasmid

#159821

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI unc-119 target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHygCG2r

Plasmid

#200074

Purpose

hygR selection mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Tags

Expression

Worm

Mutation

Promoter

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Use

Tags

Expression

Worm

Mutation

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

PU6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pTN14

Plasmid

#178945

Purpose

Expresses AtTIR1(F79G) in C elegans

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

AtTIR1(F74G)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Tags

Expression

Worm

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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Pmec-18 cz::caspase-3 (p17) [TU#814]

Plasmid

#16083

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz :: caspase-3 (p17) (CASP3 Human)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pJF6

Plasmid

#139040

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (includes STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1839

Plasmid

#154325

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter

Use

CRISPR

Tags

Expression

Worm

Mutation

F+E mutations in sgRNA

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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