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Plasmid#191064Purposeceh-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertceh-1 (ceh-1 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY69
Plasmid#191066Purposeceh-24 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)DepositorInsertceh-24 (ceh-24 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIN3_enpp-1_mScarlet
Plasmid#182934PurposeExpresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)DepositorInsertgenomic enpp-1 fused to mScarlet
UseTagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormMutationPromoterenpp-1Available sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2
Plasmid#178969PurposeExpresses miRFP2 in worm cellsDepositorInsertwmiRFP2
UseTagsnoExpressionWormMutationNOPromotertag-168 promoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS640
Plasmid#188892PurposettTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 markerDepositorInsertPmex-5::Cas9, Cbr-unc-119
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS716
Plasmid#188888PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::CreDepositorInsertfloxed Cbr-unc-119 marker and Phsp16.41::Cre
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLS1272
Plasmid#188891PurposeRic-4 sgRNA expression vectorDepositorInsertPU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIN2_mcm-3_mScarlet
Plasmid#182933PurposeExpresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)DepositorInsertgenomic mcm-3 fused to mScarlet
UseTagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormMutationPromotermcm-3Available sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIN1_GGGSx3_mScarlet
Plasmid#182936PurposeC-terminal mScarlet with flexible linker (GGGGS)x3DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM262 - pDESTR4-R3 mosTI hygroR target
Plasmid#159840PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpDESTR4-R3 mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target
Plasmid#159842PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertpDESTR4-R3 mosTI Pmlc-2_GFP target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Peft-pHCountdown-unc54
Plasmid#182304PurposeC. elegans plasmid encoding pH-Countdown with three intronsDepositorInsertpH countdown with 3 introns, with a Peft3 promoter and unc54 terminator
UseTagsExpressionWormMutationPromoterPeft-3Available sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_grh-1p-grh-1-gfp
Plasmid#177761PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14_hsp-16.2p-grh-1-gfp
Plasmid#177762PurposeOverexpression of C. elegans grh-1DepositorInsertgrh-1 (grh-1 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ157
Plasmid#163639Purposepcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertpcn-1 (pcn-1 Nematode)
UseCRISPRTagsGFPExpressionWormMutationPromoterpcn-1Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ186
Plasmid#163641Purposerps-27>DHB::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome IDepositorInsertDHB:2xmKate2::3xHA
UseCRISPRTags2xmKate2ExpressionWormMutationPromoterrps-27Available sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJH2198
Plasmid#179773PurposePrgef-1 NLF-1::GFP unc-54 3' UTR C.elegans pan-neural expression of NLF-1::GFPDepositorInsertNLF-1::GFP (nlf-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrgef-1Available sinceMarch 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pJH3137
Plasmid#179617PurposePunc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFPDepositorInsertGCaMP6
UseTagsRFPExpressionWormMutationPromoterPunc-4Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only