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pSDH61

Plasmid

#139033

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH54

Plasmid

#139052

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tbb-2 3'UTR (tbb-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF88

Plasmid

#139039

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (includes STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEM3 = ncs-1p::nCre

Plasmid

#24034

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

ncs-1 (ncs-1 Nematode)

Use

Cre/Lox

Tags

nCre

Expression

Worm

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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GFP::L4440

Plasmid

#11335

Depositor

Guy Caldwell

Insert

GFP

Use

RNAi

Tags

Expression

Worm

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pXF89

Plasmid

#139030

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag (no STOP codon, PATC introns)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCE-BiFC-VC155

Plasmid

#22020

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VC155

Use

Tags

HA

Expression

Worm

Mutation

Changed Alanine 206 to Lysine

Promoter

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKS235

Plasmid

#191855

Purpose

Negative selection marker for MosSCI

Depositor

K Subramaniam

Article

Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

Insert

peel-1 toxic protein for negative selection against extrachromosomal array

Use

Tags

Expression

Worm

Mutation

Promoter

hsp-16.41

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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Punc-4c.TIR1

Plasmid

#194053

Purpose

TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

TIR1 (TIR1 Mustard Weed)

Use

Tags

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

unc-4c

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ108 - pENTR221 - [cbr-unc-119(+)]

Plasmid

#200367

Purpose

cbr-unc-119 rescue gene for use as co-injection marker.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[cbr-unc-119(+)]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM231 - [Pmlc-1 | gfp | cbr-tbb-2 UTR]

Plasmid

#159897

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | gfp | cbr-tbb-2 UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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coel::RFP

Plasmid

#8938

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Use

Tags

RFP

Expression

Worm

Mutation

Promoter

Available since

Nov. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pGH8 - pRAB-3::mCherry::unc-54utr

Plasmid

#19359

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Prab-3::mCherry

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP670-2-T2A-HO1

Plasmid

#197250

Purpose

Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP670-2-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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TU#867 (Punc-119sid-1)

Plasmid

#25811

Depositor

Martin Chalfie

Article

Calixto et al Nat Methods. 2010 May 30. ():.

Insert

systemic RNA interference-deficient 1 (sid-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

Plasmid

#24334

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

H2B

Use

Cre/Lox

Tags

GFP

Expression

Worm

Mutation

mCherry driven by an HSP70 promoterCerulean drive…

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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