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Plasmid#1443DepositorTypeEmpty backboneUseTagsunc54enh, unc545', IVSA, rf2128ExpressionWormMutationPromoterAvailable sinceMarch 7, 2005AvailabilityAcademic Institutions and Nonprofits only
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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
XW08
Plasmid#65832PurposePunc-4-QF-SL2-mcherry-3'UTR-unc-54DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationPromoterPunc-4Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJH2685
Plasmid#191372PurposePunc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFPDepositorInsertTetanus Toxin Light Chain
UseTagswCherryExpressionWormMutationPromoterPunc-4Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIN4_tsp-6_mScarlet
Plasmid#182935PurposeExpresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)DepositorInsertPtsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)
UseTagsflexible linker (GGGGS)x3 and mScarlet tagExpressionWormMutationPromotertsp-6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS3-lim-4P_gtACR2_SL2_eGFP(65C)_unc-54 3'UTR
Plasmid#195853PurposeExpresses inhibitory opsin gtACR2 and GFP in turning associated neuronsDepositorInsertGuillardia theta anion-conducting channelrhodopsin-2 (GtACR2)
UseTagsExpressionWormMutationPromoterlim-4Available sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::tagBFP
Plasmid#124343PurposeNuclear Localized Pan-Neuronal tagBFPDepositorInsertrab-3, tagBFP (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2
Plasmid#182353PurposeGFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1259
Plasmid#61251PurposeCas9 plasmid with sgRNA deleted; for use in C. elegansDepositorInsertDeletion
UseCRISPRTagsExpressionWormMutationDeleted sgRNA(F+E) sequence from pJW1219PromoterAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST- IP2.0(codon for C.elegans)
Plasmid#109216PurposeIP2.0 for C. elegans (Codon optimization and introns).DepositorInsertIP2.0 (Inverse Pericam 2.0)
UseTagsHis-tagExpressionWormMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS051
Plasmid#137025PurposeSapTrap mScarlet-I-GLO donorDepositorInsertC. elegans GLO-optimized mScarlet
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized mScarletExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
punc-119cR
Plasmid#21596DepositorInsertunc-119:mCherry (unc-119 Nematode)
UseAdds unc-119 marker to amp resistant plasmidsTagsmCherryExpressionWormMutationN/APromoterAvailable sinceAug. 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMLS328
Plasmid#73717PurposeC. elegans germline CRE expression vectorDepositorInsert2xNLS-CRE
UseCre/LoxTagsExpressionWormMutationPromoterPeft-3Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1-odr-4P::his-24::mNeptune
Plasmid#124342PurposeMulticolor Worms for Neuronal IDDepositorInsertodr-4, mNeptune (odr-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW2088
Plasmid#154336PurposeCrispr repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::GFP::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHygRR2
Plasmid#200075PurposehygR selection mScarlet 2X marked rapid RMCE integration vectorDepositorTypeEmpty backboneUseRecombination mediated cassette exchangeTagsExpressionWormMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only