1,779 results

1. pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

   Plasmid

   #159811

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Expression

   Worm

   Promoter

   eft-3

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMB66

   Plasmid

   #47946

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Promoter

   hsp-16.48

   Available Since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Pmex-5

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSO221 Phis-72 BirA GFP

   Plasmid

   #45100

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   BirA::GFP
   unc-119 (unc-119 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   his-72 and unc-119

   Available Since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSDH61

   Plasmid

   #139033

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (no STOP codon)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD288

   Plasmid

   #137017

   Purpose

   mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   mNG-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized mNG

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK73.ChrII_ttTi5605.sgRNA

   Plasmid

   #194058

   Purpose

   sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrII_ttTi5605 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK73.ChrV_oxTi365.gRNA

   Plasmid

   #194052

   Purpose

   sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ChrV_oxTi365 sgRNA
   

   Expression

   Worm

   Promoter

   U6

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ1655

    Plasmid

    #51481

    Purpose

    miniMos transposable element with HygroR, R4-R3 Gateway cloning

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS1-lim-4P::his-24::Cerulean

    Plasmid

    #124336

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    lim-4, Cerulean (lim-4 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ150-set2

    Plasmid

    #82546

    Purpose

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR construct used for microinjection

    Depositor

    Sean Ryder

    Article

    Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

    Insert

    Pmex-5:GFP::H2B::PEST::set-2 3'UTR
    

    Expression

    Worm

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS716

    Plasmid

    #188888

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. XW08

    Plasmid

    #65832

    Purpose

    Punc-4-QF-SL2-mcherry-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Tags

    mCherry

    Expression

    Worm

    Promoter

    Punc-4

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159854

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSM_ser-2prom3p::myr-mCherry

    Plasmid

    #164223

    Purpose

    Expression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.

    Depositor

    Yan Zou

    Article

    Feng et al Cell Rep. 2020 Sep 8;32(10):108101. doi:

    Insert

    myr-mCherry
    

    Expression

    Worm

    Promoter

    ser-2promo3

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW1582

    Plasmid

    #154312

    Purpose

    mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mNeonGreen^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJW1259

    Plasmid

    #61251

    Purpose

    Cas9 plasmid with sgRNA deleted; for use in C. elegans

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    Deletion
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Deleted sgRNA(F+E) sequence from pJW1219

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1584

    Plasmid

    #121055

    Purpose

    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    YPET^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, C. elegans codon optimized YPET, and auxi…

    Expression

    Worm

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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