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Plasmid#194969PurposeFluorescent reporter of mitochondrial matrix Ca2+.DepositorInsert4 repeats of Cox8 presequence plus GCaMP6f
UseTagsExpressionWormMutationPromoterFlp-18Available sinceJune 21, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJJR81
Plasmid#75029PurposetagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInserttagBFP2^SEC^3xFlag
UseCRISPRTags3xFLAG and C. elegans codon optimized tagBFP2ExpressionWormMutationPromoterAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJA281
Plasmid#22513DepositorInsertPmex-5::mCherry (w introns w/o stop)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMLS134
Plasmid#73714PurposeSapTrap destination vector for building sgRNA expression vectors.DepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJA327
Plasmid#74486Purposesuperfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)DepositorInsertsuperfolder GFP
UseTagsExpressionWormMutationPromotermyo-3Available sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAH71 - [AWAp | wrmScarlet | tbb-2 UTR]
Plasmid#200345PurposemScarlet BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH72 - [AWAp | gfp | tbb-2 UTR]
Plasmid#200346Purposegfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
PU6::klp-12_sgRNA
Plasmid#46170DepositorInsertklp-12 targeting sgRNA (klp-12 Synthetic)
UseCRISPRTagsExpressionWormMutationPromoterC. elegans U6 snRNA pol III promoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJW2072
Plasmid#154334PurposeCrispr repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
UseCRISPRTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1838
Plasmid#154324PurposesgRNA Destination Vector for SapTrapDepositorInsertSapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNM3732
Plasmid#153086Purposemec-4p GFP-C1 positive control for RMCE integrationDepositorInsertmec-4p GFP-C1 tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]
Plasmid#159896PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW2172
Plasmid#163096PurposeCRISPR repair template: add insertion site specific homology arms by PCR before insertionDepositorInsert30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationSilent mutations to remove piRNA sitesPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
XW09
Plasmid#65833PurposePunc-4c-QS-SL2-mcherry3'UTR-unc-54DepositorInsertNeurospora crassa OR74A repressor (qa-1S)
UseTagsmCherryExpressionWormMutationPromoterPunc-4cAvailable sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW6.01
Plasmid#62882PurposeTransgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTRDepositorInsertGFP::MEG-3
UseTagsGFPExpressionWormMutationPromotermeg-3Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1584
Plasmid#121055PurposeYPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.DepositorInsertYPET^SEC^AID*::3xFlag
UseCRISPR and Cre/LoxTags3xFLAG, C. elegans codon optimized YPET, and auxi…ExpressionWormMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmCardinal
Plasmid#197251PurposeExpresses the protein of wmCardinal in neurons of C. elegansDepositorInsertwmCardinal
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xHA-BioID_pAS28
Plasmid#118223Purposeexpresses 3xHA-tagged BioID in C. elegans intestineDepositorInsertBioID (BirA mutant)
UseTags3x HA tagExpressionWormMutationR118GPromoterges-1pAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only