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Plasmid#200073PurposehygR selection mNG marked rapid RMCE integration vectorDepositorTypeEmpty backboneUseRecombination mediated cassette exchangeTagsExpressionWormMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCFJ1955
Plasmid#84830PurposeMinimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized tdTomato
UseTagsExpressionBacterial and WormMutationPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEft-3::oxCerulean-oxVenus::Lgg-1
Plasmid#68040PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagyDepositorInsertpEft-3::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS050
Plasmid#91826PurposeCodon-optimized mScarlet-I donor for the SapTrap cloning systemDepositorInsertmScarlet-I-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH2217
Plasmid#179251PurposePsra-11::TWK-18(gf)-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of TWK-18-wCherryDepositorInsertTWK-18 (twk-18 Nematode)
UseTagswCherryExpressionWormMutationM280IPromoterPsra-11Available sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxTagsExpressionWormMutationHYGR∆ encodes aa 1-226PromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMLS291
Plasmid#73724PurposeSapTrap mCherry + Cbr-unc-119 donor plasmidDepositorInsertmCherry + Cbr-unc-119
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
Plasmid#159811PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]
Plasmid#66753PurposeminiSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondriaDepositorInsertminiSOG
UseTagstomm-20 (N terminal 55 aa)ExpressionWormMutationPromoterAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRB1080
Plasmid#71309PurposeExpresses VQR Cas9 variant with eef-1A.1 (eft-3) promoterDepositorInsertVQR Cas9 variant
UseCRISPRTagsExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD04neo pmyo2::gfp
Plasmid#26393DepositorTypeEmpty backboneUseTagsExpressionBacterial and WormMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDD362
Plasmid#137023PurposeSapTrap HaloTag-GLO donorDepositorInsertC. elegans GLO-optimized Halo Tag
UseCRISPR; Saptrap donor for cloning repair templat…TagsC. elegans GLO-optimized Halo TagExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ70 - Pmyo-3::twk-18(cn110)::unc-54utr
Plasmid#19326DepositorArticleInsertPmyo-3::twk-18(cn110)cDNA
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAS1 - pRig-3::GCaMP6f::unc-54
Plasmid#194970PurposeFluorescent reporter of cytosolic calcium in C.elegans.DepositorInsertGCaMP6f
UseTagsExpressionWormMutationPromoterrig-3Available sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY22
Plasmid#191028Purposelim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertlim-4 (lim-4 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY17
Plasmid#191023Purposeflp-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-18 CELE_Y48D7A.2 (flp-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY16
Plasmid#191022Purposeflp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-6 (flp-6 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY18
Plasmid#191024Purposeflp-19 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-19 (flp-19 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY19
Plasmid#191025Purposeflp-26 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-26 (flp-26 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY21
Plasmid#191027Purposeggr-3 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertggr-3
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only